Expression of Amaranthus viridis L. Agglutinin Mediated by Matrix Attachment Region (MAR) Sequence in Transgen ic Chinese Cabbage

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (2): 381-386.

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Expression of Amaranthus viridis L. Agglutinin Mediated by Matrix Attachment Region (MAR) Sequence in Transgen ic Chinese Cabbage

DENG Zhi-nian^1,2*, WEI Yuan-wen^1,2, LüWei-li¹,and LI Yang2rui¹

(¹Key Laboratory of Crop Genetic Im provem ent and Biotechnology of Guangxi, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; ²Agricultural College, Guangxi University, Nanning 530004, China )

Received:2006-08-14 Revised:2007-01-25 Online:2007-04-25 Published:2007-04-25
Contact: DENG Zhi-nian

Abstract

Abstract: The cotyledonswith petiole as acceptors, Chinese cabbage genetic transformation system mediated by Agrobacterium tum efaciens was set up and Amaranthus viridis L. agglutin in (AVA ) transformed plants resistanting to Myzus persicae were acquired. The effects of MAR (matrix attachment region) on AVA gene exp ression in transgentic Chinese cabbage were studied with two different p lant exp ression vectors. The number of transgenic plants was 29163% higher than that of the control. The bioassay of Myzus persicae revealed that transgetic plants showed more or less enhanced resistance and inhibition to population, and the average inhibition rate was 55.8%. Among the transgetic plants that AVA gene were mediated by MAR, the expression level of AVA gene was higher than that of the control, and the expression variance of AVA gene among transgetic p lantswas smaller as well.

Key words: Chinese cabbage, Matrix attachment regions, AVA gene, Myzus persicae, Transgene expression

CLC Number:

S 634.3

DENG Zhi-nian;WEI Yuan-wen;LüWei-li;and LI Yangrui. Expression of Amaranthus viridis L. Agglutinin Mediated by Matrix Attachment Region (MAR) Sequence in Transgen ic Chinese Cabbage[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 381-386.

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Expression of Amaranthus viridis L. Agglutinin Mediated by Matrix Attachment Region (MAR) Sequence in Transgen ic Chinese Cabbage

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