Studies on Transformation of hrap Gene in to Tomato

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (2): 377-380.

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Studies on Transformation of hrap Gene in to Tomato

WANG Shui-qi^1,2,CHEN Jian^1,3,and LIN Zhong-ping¹

(¹The National Key Laboratory of Protein Engineering and Plant Genetic Engineering, Pek ing University, Beijing 100087, China; ² Zhangzhou Institute of Technology, Zhangzhou, Fujian 363000, China; ³Fujian Academy of Agricultural Sciences,Fuzhou 350003, China)

Received:2006-07-20 Revised:2006-11-23 Online:2007-04-25 Published:2007-04-25

Abstract

Abstract: Sweet pepper hrap gene under the control of CaMV 35S promoter has been transformed into tomato via Agrobacterium tumefaciens mediated procedure. Plantletswere regenerated onMSmedium with 3.0mg·L^{- 1} 6-BA, 0.2 mg·L^-1 IAA, and 50 mg·L^-1 kanamycin1 The transgenic plants had been identified by
PCR procedure and Southern blot, and their exp ression was showed by Northern hybridization. A preliminary assay with leaf disc infected by tomato pathogen Ralstonia solanacea rum Smith showed that transgenic plants
had higher resistance to the pathogen than wild2type tomato.

Key words: Tomato, Genetic transformation

CLC Number:

S 641.2

WANG Shui-qi;;CHEN Jian;and LIN Zhong-ping. Studies on Transformation of hrap Gene in to Tomato[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 377-380.

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Studies on Transformation of hrap Gene in to Tomato

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