Development of SSR Markers in Sweet Cherry Using Selectively Amplified Microsatellite

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (2): 311-316.

Development of SSR Markers in Sweet Cherry Using Selectively Amplified Microsatellite

AI Cheng-xiang¹, YU Xian-mei², ZHANG Li-si¹, WE IHai-rong¹, XIN Li¹, YUAN Ke-jun¹, JIN Song-nan¹,SUN Qing-rong¹, and LIU Qing-zhong^1*

(¹Shandong Institute of Pom ology, Key Laboratory for Fruit Biotechnology Breeding of Shandong, Taipan, Shandong 271000,China; ²Institute of Environm ent and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Danzhou,Hainan 571737, China)

Received:2006-07-08 Revised:2006-12-26 Online:2007-04-25 Published:2007-04-25
Contact: LIU Qing-zhong

Abstract

Abstract: A total of 100 sequenceswere isolated and cloned by SAM ( SelectivelyAmp li2
fiedMicrosatellite) and another one was obtained from the NCBI and EMBL databases. 82 SSR sequenceswere used to design the special primers at 77 loci from 69 fragments. Thirty-eight pairs of special primerswere synthesized, matchingwith 5′anchored degenerate SSR p rimer, to detect 36 SSR loci. 27 p rimer pairs amp lified clear and robust DNA fragment, of which nineteen pairs of SSR p rimers amp lified the corresponding SSR sequences and eight amplified the unexpected fragments. 24 polymorphic p rimer pairs were selected from the 27 p rimer pairs by using the genomic DNA of 27 sweet cherry germp lasm, and 24 locus-specific SSR markerswere obtained.

Key words: Prunus avium L., Germplasm, SSR markers, Selectively Amplified Microsatellite

CLC Number:

S 662.5

AI Cheng-xiang;YU Xian-mei;ZHANG Li-si;WE IHai-rong;XIN Li;YUAN Ke-jun;JIN Song-nan;SUN Qing-rong;and LIU Qing-zhong. Development of SSR Markers in Sweet Cherry Using Selectively Amplified Microsatellite[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 311-316.

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Development of SSR Markers in Sweet Cherry Using Selectively Amplified Microsatellite

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