AFLP Markers of Cucumber Anthracnose Resistance-related Gene

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (1): 213-216.

• 研究简报 • Previous Articles Next Articles

AFLP Markers of Cucumber Anthracnose Resistance-related Gene

WANG Hui-zhe¹; LI Shu-ju¹; LIU Xiu-feng²; LI Ping¹; HUO Zhen-rong¹; GUAN Wei¹

(¹ Tianjin Kernel Cucumber Research Institute, Tianjin 300192, China; 2College of Chemistry, Nankai University, Tianjin300071, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

Abstract

Abstract: With a F₂ population derived from 66 ×A18 asmaterials, polymorphism between resistant and
suscep tible bulk of cucumber anthracnose were studied using BSA method and AFLP technology. A codomi
nantAFLP marker, E24M48=251 bp /245 bp, was screened. The resistant and suscep tible F₂ plants possessed
the 251 bp and the 245 bp fragment, respectively, and the mid-plants owned the two fragments. The AFLP
markerwas closely linked with the cucumber anthracnose resistance related gene, and the genetic distance be
tween the marker and gene was 21727 cM. Fragements sequencing of the codominantAFLP marker indicated
that their length were 251 bp and 245 bp, respectively. The difference of the two fragements lied in the insert
deletion of two “TCT” repeat sequences.

Key words: Cucumber, Anthracnose, Resistance-related gene, AFLP

WANG Hui-zhe;LI Shu-ju;LIU Xiu-feng;LI Ping;HUO Zhen-rong;GUAN Wei. AFLP Markers of Cucumber Anthracnose Resistance-related Gene[J]. ACTA HORTICULTURAE SINICA, 2007, 34(1): 213-216.

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AFLP Markers of Cucumber Anthracnose Resistance-related Gene

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