The Construction and the Chilling-Resistance Ability of Endoplasmic Reticulum Small Heat Shock Protein ( ER-sHSP) Transgenic Tomato Plants

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (5): 989-994.

• 研究论文 • Previous Articles Next Articles

The Construction and the Chilling-Resistance Ability of Endoplasmic Reticulum Small Heat Shock Protein ( ER-sHSP) Transgenic Tomato Plants

Zhao Chunmei;Wang Li;Yi Shuying;Liu Jian^*

(College of Life Sciences, Shandong Normal University, Ji'nan, Shandong 250014, China)

Received:2006-04-14 Revised:2006-09-20 Online:2006-10-25 Published:2006-10-25

Abstract

Abstract: The full-length endoplasmic reticulum small heat shock protein ( ER-sHSP) cDNA using CaMV 35S p romoterwas transformed into the genome of tomato plants. The chilling tolerance of transgenic tomato plants, non2transgenic tomato plants and pROKⅡ-transformed tomato plants were studied. Under chilling stress treatment, compared with non-transgenic and pROKⅡ-transformed tomato plants, transgenic tomato plants exhibited slighter cold-injured symptoms, suffered less destruction of chlorophyll and electrolyte leakage. The content of MDA of transgenic tomato plants was lower than that of non-transgenic and pROKⅡ-transformed tomato plants, and transgenic tomato plants could keep up higher value of net photosynthetic rate, Fv/Fm and could recover quickly from chilling-induced photosynthetic inhibition. The results showed that ERsHSP played a key role in enhancing the chilling-resistance ability of plants.

Key words: Tomato, Endoplasmic reticulum, Small heat shock protein, Transgenic, Chilling stress

Zhao Chunmei;Wang Li;Yi Shuying;Liu Jian. The Construction and the Chilling-Resistance Ability of Endoplasmic Reticulum Small Heat Shock Protein ( ER-sHSP) Transgenic Tomato Plants[J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 989-994.

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The Construction and the Chilling-Resistance Ability of Endoplasmic Reticulum Small Heat Shock Protein ( ER-sHSP) Transgenic Tomato Plants

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