Cloning and Expression of β-galactosidase Gene cDNA in Peach

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (4): 721-724.

• 研究论文 • Previous Articles Next Articles

Cloning and Expression of β-galactosidase Gene cDNA in Peach

Bian Weihua^{1, 4};Jiang Changjun2*;Wang Zhaoxia³

( ¹ Key Laboratory of Tea Biochemistry & Biotechnology, Anhui Agricultural University, Hefei, Anhui 230036, China;²Biotechnology Center, Anhui Agricultural University, Hefei, Anhui 230036, China; ³Department of Biology, Anhui Institute of education, Hefei, Anhui 230061, China; ⁴Department of Biochemistry, Binzhou Medical College, Binzhou, Shandong 256603, China)

Received:2005-06-18 Revised:2005-10-11 Online:2006-08-25 Published:2006-08-25

Abstract

Abstract: A 3 285 bp cDNA encoding β-galactosidase was cloned from compress peach by the methods of RT-PCR and rapid amplification of cDNA ends (RACE). The accession number is AY874412, which contains 2 559 bp, encoding a 853 residue polypeptide. The cDNA was constructed into the vector pET-32a( + ) designed for recombinant strain. After IPTG induction, it was observed there was a special band about 115 kDa on SDS-PAGE. The activity of the fusion protein was analyzed by in vitro enzymatic reaction.

Key words: Peach, β-galactosidase, Rapid amplification of cDNA ends, cDNA cloning, Prokaryotic expression

Bian Weihua;;Jiang Changjun;Wang Zhaoxia. Cloning and Expression of β-galactosidase Gene cDNA in Peach[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 721-724.

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Cloning and Expression of β-galactosidase Gene cDNA in Peach

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