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ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (3): 635-638.

• 研究简报 • Previous Articles     Next Articles

In Vitro Selection of Salt-tolerant Mutant from Potato Stem Explants

Cheng Zhihui*;Li Juan;Zhang Guoyu   

  1. (College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China)
  • Received:2005-08-22 Revised:2005-10-24 Online:2006-06-25 Published:2006-06-25

Abstract: The stem explants of four potato cultivarswere used to induce calli and to screen salt-tolerant mutants in the medium containing different levels of NaCl. The results indicated that different cultivars showed different tolerance levels. To calli induction, the highest salt tolerant level is 018% for cv. Dongnong 303 and E1, 0.6% for cv. Favorite and 0.4% for cv. Shepody. Salt inhibited shoot and root differentiation. The salt-tolerant calli should be subcultured for notmore than 8 - 10 times. Salt tolerant tests in vivo and in vitro revealed that the mutants possessed salt tolerance.

Key words: Potato, Salt-tolerant mutant, Stem explants, Callus, Culture in vitro