Abstract: Factors affecting in vitro leaf regeneration including age of leaf, source of leaf, condition of tube-plantlet and hormones in culture medium were studied in Ziziphus jujuba ‘Huanghua Dongzao’. A high-efficient system of in vitro leaf regeneration was established. The upper and middle leaves from rootless tube-plantlet were easier to regenerate than those from rooted tube-plantlet. The efficiency of TDZ was significantly higher than BA in the induction of adventitious bud from leaf. Leaves should be first induced on MS medium
supplemented with TDZ (1.0 mg·L ^{- 1} ) and IBA (0.1 mg·L ^{- 1} ) for 28 days, and than transferred to medium MS + IBA 0.1 mg·L ^{- 1} + GA₃ 0.05 mg·L ^{- 1}. In this way, the regeneration rate reached 92.45%. MS + BA 1.0 mg·L ^{- 1} + KT 0.5 mg·L ^{- 1} + IBA 0.1 mg·L ^{- 1} was suitable for subculture of shoots, with the multiplication coefficient of 3.64. The regenerated plantlets rooted well in 1 /2MS medium plus IAA (1.0 mg·L ^{- 1} ) , with rooting percentage of 87.1%. The survival rate of rooted plantlets in the field was 57%.

Key words: Chinese jujube, Leaf culture, Plantlet regeneration