Abstract: This experiment was conducted to investigate in vitro microp ropagation and regeneration of petiole, leaf disk and internodal segments in ground cover rose (Rosa hybrida L. ) ‘Royal Bassino’. The results showed that using axillary as explants, the optimum induction medium with 100% of shoots rate was basal
MS supplemented with 6-BA 1.5 mg/L and IBA 0.1 mg/L; and the highest shoot proliferation rate of 6.3 timeswas obtained on the medium of MS + 6-BA 0.5 mg/L + IBA 0.05 mg/L; and 1/2MS with IBA 0.01 mg/L was the optimum shoot rooting medium with 92% rooting. After 7 d of acclimation, the plantlets were
transplanted to a greenhouse, and 99% survivals were obtained. Using petiole as explants for regeneration culture, derived either from loose callus or through organogenesis, the rate of adventitious buds was reached to 57.1% through following procedures: dark-induced initially for 10 d on the medium of MS + thidiazuron ( TDZ) 7μmol/L, then cultured on the medium of MS + 6-BA 0.5 mg/L for about 20 d under photoperiod of 16 /8 h. However, no successful regenerations of adventitious buds were observed in the culture using leaf
disks and internodal segments.

Key words: Rose, In vitro micropropagation, Regeneration