Expression of Human Lactoferr in Gene in Transgenic Tomato Plants

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (6): 1030-1033.

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Expression of Human Lactoferr in Gene in Transgenic Tomato Plants

Cao Huiying;Guo Sandui

(Biotechnology Research Institute, the Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Received:2005-03-04 Revised:2005-04-22 Online:2005-12-25 Published:2005-12-25

Abstract

Abstract: The plant expression vector pBI121LF contained artificial synthesized human lactoferrin gene was constructed and introduced into tomato by Agrobacterium -mediated. Twenty-three regenerated plants were obtained through kanamycin-resistant selection. Itwas demonstrated that lf gene had been integrated into tomato genome by PCR and Southern blot analysis. Western blot analysis showed that human lactoferrin had expressed in transgenic tomato fruits. Itmade the basic research for transgenic tomato as healthy food.

Key words: Lactoferrin, Transgenic, Tomato, Bioreactor

CLC Number:

S 641.2

Cao Huiying;Guo Sandui. Expression of Human Lactoferr in Gene in Transgenic Tomato Plants[J]. ACTA HORTICULTURAE SINICA, 2005, 32(6): 1030-1033.

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Expression of Human Lactoferr in Gene in Transgenic Tomato Plants

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