Abstract: Research on expression of different periods and sites was based on gene cloning. The result showed that cs-acs1g gene did not exp ress during 1 - 5 leaves without treatment of hormone, as the same time, it showed the same consequence under treatment of ETH and GA, cs-acs1g gene would exp ress at the period of two leaves with treatment of certain concentration of IAA, the intensity was accumulated with increasing frequent of IAA; cs-acs1g gene would transcript differently at various sites, the intensity which the root tips and stem and tip's represent was distinctlymore strong than that of fruit and axillary buds. therefore,it existed discrepancy at different periods and various sites.

Key words: Cucumber, Gene, Gynoecium, Hormone, Periods and sites expression