C-banding of Prometaphase Chromosomes in Cucumber and the Procedure for Slide Preparation

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (6): 814-816.

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C-banding of Prometaphase Chromosomes in Cucumber and the Procedure for Slide Preparation

Qian Chuntao; Chen Jinfeng^*;Luo Xiangdong; Cao Qinghe;Lei Chun

( State Key Laboratory of Crop Genetics and Germplasm Enhancement , College of Horticulture , Nanjing Agricultural University , Nanjing 210095 , China)

Received:2004-02-02 Revised:2004-04-05 Online:2004-12-25 Published:2004-12-25

Abstract

Abstract: Clear C-bands at prometaphase were obtained in cucumber ( Cucumis sativus L. , 2n = 2x = 14) when cycloheximide was applied to the germinating roots in vivo as pretreatment . Chromosome length at prometaphase varied from 3 to 8μm. Haploid complement had 21 distinct and stable C-bands , including 12 telomeric - , 7 centromeric - , 1 intercary - and 1 satellite-bands. Some factors affecting the band formation were investigated and a practical procedure of C-banding of prometaphase chromosome was proposed.

Key words: Cucumber, Chromosome, C2banding, Prometaphase

CLC Number:

S 642.2

Qian Chuntao;Chen Jinfeng;Luo Xiangdong;Cao Qinghe;Lei Chun. C-banding of Prometaphase Chromosomes in Cucumber and the Procedure for Slide Preparation[J]. ACTA HORTICULTURAE SINICA, 2004, 31(6): 814-816.

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C-banding of Prometaphase Chromosomes in Cucumber and the Procedure for Slide Preparation

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