Improvement of Genetic Transformation System by Using GFP as ReporterGene on Pepper

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (6): 737-742.

• 研究论文 • Previous Articles Next Articles

^{Improvement of Genetic Transformation System by Using GFP as ReporterGene on Pepper}

Yang Guoshun^1,2;Xie Bingyan^1*; Yang Yuhong¹; Wang Xiaowu¹; Lu Xiangyang²; Jiang Fangling^1,2

(¹ Institute of Vegetables and Flowers , Chinese Academy of Agricultural Science , Beijing 100081 , China ; ² Horticultural and Landscape College , Hunan Agricultural University , Changsha 410128 , China)

Received:2003-12-02 Revised:2004-03-24 Online:2004-12-25 Published:2004-12-25
Contact: Xie Bingyan

Abstract

Abstract: Effect of transferring efficiency of Agrobacterium tumef aciens pH value 、concentration of AS added in co-cultivation medium , temperature during co2cultural stage and days for co-cultivation were studied through PCR
amplification and observation of green fluorescent protein expression by used GFP as reporter gene on cotyledon of ‘Zhongjiao 5’and‘Xiangyan 10’. The results show that when pH value of Agrobacterium solution is 5.2 , transformation efficiency of pepper cotyledon incubated on co-cultivation medium added 200μmol·L ^-1 AS increased significantly under 23 ℃and 5 days dark co-cultivation. The rate of expression of green fluorescent protein are up to 40 % on calli of cotyledon of both‘Zhongjiao 5’and‘Xiangyan 10’.

Key words: Pepper, Cotyledon, Green fluorescent protein, Genetic transformation

CLC Number:

S 641.3

Yang Guoshun;Xie Bingyan;Yang Yuhong;Wang Xiaowu;Lu Xiangyang;Jiang Fangling;. ^{Improvement of Genetic Transformation System by Using GFP as ReporterGene on Pepper}[J]. ACTA HORTICULTURAE SINICA, 2004, 31(6): 737-742.

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^{Improvement of Genetic Transformation System by Using GFP as ReporterGene on Pepper}

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