Protocol Establ ishment of Tall Fescue Transgene by Biolistics

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (5): 691-693.

• 研究报告 • Previous Articles Next Articles

Protocol Establ ishment of Tall Fescue Transgene by Biolistics

Ma Shengjian^1,3;Zeng Fuhua^1*; Xu Biyu²; Lu Xiangyang^3*;Wu Zhihua^1,3

(¹ Department of Biology , Zhanjiang Normal College,Zhanjiang 524048, China; ² The State Key L aboratory of Tropical Crops Biotechnology,Chinese Academy of Tropical Agricultural Sciences , Haikou 571101,China;³ Department of Biotechnology , Hunan Agricultural University , Changsha 410128 , China)

Received:2004-02-02 Revised:2004-04-22 Online:2004-10-25 Published:2004-10-25

Abstract

Abstract: In this paper , excised mature embryos of tall fescue were used as explants to induce embryonic calli , which were used as materials to optimize bombardment parameters of biolistics , and the most suitable delivery parameters for PDS-1000/ He were 6 cm shoot distance , 6 cm distance between particles and rupture disks , 1100 psi helium pressure and ombarding 2 shots. An optimized t ransformation protocol was established and transgenic turfgrass varieties resistant to herbicide with stable heredity were acquired.All tested tall fescues selected randomly were positive by PCR analysis and by dot blotting analysis for bar gene. The results of daubing tests with herbicide showed t ransgenic plants had more resistance than plants
cont rol did.

Key words: Turfgrass, Callus, Biolistics, Transformation, Herbicide resistance

CLC Number:

S 68

Ma Shengjian;Zeng Fuhua;Xu Biyu;Lu Xiangyang;Wu Zhihua;. Protocol Establ ishment of Tall Fescue Transgene by Biolistics[J]. ACTA HORTICULTURAE SINICA, 2004, 31(5): 691-693.

[1]	ZHANG Wanqing, ZHANG Hongxiao, LIAN Xiaofang, LI Yuying, GUO Lili, HOU Xiaogai. Analysis of DNA Methylation Related to Callus Differentiation and Rooting Induction of Paeonia ostii‘Fengdan’ [J]. Acta Horticulturae Sinica, 2022, 49(8): 1735-1746.
[2]	QIU Keli, WANG Yumin, HE Jinling, YU Hong, PAN Haifa, SHENG Yu, XIE Qingmei, CHEN Hongli, ZHOU Hui, ZHANG Jinyun. Identification of Peach Laccase Family Genes and Function Analysis of PpLAC21 [J]. Acta Horticulturae Sinica, 2022, 49(6): 1351-1362.
[3]	LI Yamei, MA Fuli, ZHANG Shanqi, HUANG Jinqiu, CHEN Mengting, ZHOU Junyong, SUN Qibao, SUN Jun. Optimization of Jujube Callus Transformation System and Application of ZjBRC1 in Regulating ZjYUCCA Expression [J]. Acta Horticulturae Sinica, 2022, 49(4): 749-757.
[4]	LIU Rong, CI Huiting, REN Xiuxia, GAO Jie, WANG Shunli, ZHANG Xiuxin. Optimization of Callus Induction from Immature Embryo and Establishment Regeneration System of Paeonia ostii‘Fengdan’ [J]. Acta Horticulturae Sinica, 2022, 49(1): 166-174.
[5]	SU Liyao, WANG Peiyu, JIANG Mengqi, HUANG Shuqi, XUE Xiaodong, LIU Mengyu, XIAO Xuechen, LAI Chunwang, ZHANG Zihao, CHEN Yukun, LAI Zhongxiong, LIN Yuling. The Activity Verification of pri-miR319a Encode Regulatory Peptide of Dimocarpus longan [J]. Acta Horticulturae Sinica, 2021, 48(5): 908-920.
[6]	LUO Hongyu, YANG Jiangwei, FENG Ya, ZHANG Huanhuan, LIU Shengyan, ZHANG Ning, SI Huaijun. The Effect of Stu-miR156 Silencing by STTM Technology on Potato Lateral Root Development [J]. Acta Horticulturae Sinica, 2021, 48(3): 531-538.
[7]	LIU Yajie, LIANG Chen, WANG Li, DU Guoqiang, SHI Xiaoxin. Analysis of Proliferation Abilities and Telomere Lengths of Apple Plantlets in Vitro Preserved for Eight to Thirty-three Years [J]. Acta Horticulturae Sinica, 2021, 48(2): 243-253.
[8]	FU Yongyao1，Liu Jianling1，ZHU Yiyong1，XU Wenji1，Lei Meiyan2，and YANG Liping1,*. Construction of Transformation System and Integration of LrCCoAOMT Gene into Lilium lancifolium [J]. ACTA HORTICULTURAE SINICA, 2020, 47(7): 1345-1358.
[9]	YAN Siyuan, DU Juan, YANG Fulong, REN Miaomiao, LI Jiahong, and GU Peiwen. Establishment of Genetic Transformation System of Lycium barbarum Endophytic Fungus Fusarium nematophilum NQ8GⅡ4 Using PEG-mediated Method and Transformants Evaluation [J]. Acta Horticulturae Sinica, 2020, 47(12): 2385-2396.
[10]	XIE Xingnan，YANG Li，LIU Fan，TIAN Na，CHE Jingru，JIN Sanpeng，ZHANG Yongyan，and CHENG Chunzhen. Establishment and Optimization of Valencia Sweet Orange in planta Transformation System [J]. ACTA HORTICULTURAE SINICA, 2020, 47(1): 111-119.
[11]	CUI Huilin1,2，LI Zhiyuan2，FANG Zhiyuan2，YANG Limei2，ZHUANG Mu2，Lü Honghao2，LIU Yumei2，SONG Jianghua1,，and ZHANG Yangyong2,. Establishment and Application of YL-1 High-efficiency Genetic Transformation System in Cabbage（Brassica oleracea L. var. capitata） [J]. ACTA HORTICULTURAE SINICA, 2019, 46(2): 345-355.
[12]	LIU Hongjiu1，WEN Yanbin1，LIU Xiaoxue1，ZHAO Yongqiang1，HUANG Caiping2，and CHENG Zhihui1,*. A Review for Molecular Biology of Allium sativum [J]. ACTA HORTICULTURAE SINICA, 2018, 45(9): 1778-1790.
[13]	ZHANG Ruiteng1,2，Lü Jianchun1，ZHOU Mengdi1，LIU Jinglin3，LI Ren3，GUO Yangdong3，WANG Huaisong1,，and FU Qiushi1,. Expression and Function Analysis of CmGAS1 in Melon [J]. ACTA HORTICULTURAE SINICA, 2018, 45(10): 1929-1940.
[14]	DONG Jing，WANG Guixia，ZHONG Chuanfei，CHANG Linlin，SUN Jian，ZHANG Hongli，SUN Rui，SHI Kun，WEI Yongqing，and ZHANG Yuntao*. Studying Function of Alcohol Acyltransferase Gene FvAATW2 of Fragaria vesca by Over-expressing in Tobacco and Cultivated Strawberry [J]. ACTA HORTICULTURAE SINICA, 2018, 45(1): 41-50.
[15]	LIU Xiaofen，XIANG Lili，YIN Xueren，LI Fang，and CHEN Kunsong*. Ethylene Responsive Factors ERF Regulated the Hypoxia Response of Transformed Chrysanthemum Lines [J]. ACTA HORTICULTURAE SINICA, 2018, 45(1): 109-116.

Protocol Establ ishment of Tall Fescue Transgene by Biolistics

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments