Abstract: To initially study the role of Nramp gene in resisfng iron deficiency stress，we analyzed this gene in Malus xiaojinensis Cheng et Jiang by using blot hybridization technique with Nramp gene fragment of wheat as probe．Southern blot result suggested that there was Nramp gene in the genome of Malus xiaojinensis.Northern blot confirmed that transcription ofthe Nramp gene is induced in both roots and leaves and strengthened in leaves by iron stress.A 752 bp fragment of Nramp gene was obtained by PCR from the genomic DNA，
which has 83％ identities to OsNramp3 of rice in amino acid leve1．The results lead to the prediction that Nramp gene of Malus xiaojinensis may play a crucial role in resisting iron stress．

Key words: Malus xiaojinensis Cheng et Jiang, Iron, Nramp, Blot