Plant Regeneration from the Cryopreserved Somatic Hybrid of Citrus

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (2): 215-216.

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Plant Regeneration from the Cryopreserved Somatic Hybrid of Citrus

Wang Zicheng;Deng Xiuxin

(The National Key Laboratory ofCrop Genetic Improvement，Huazhong Agricultural University，Wuhan 430070，China)

Received:2003-05-27 Revised:2003-07-28 Online:2004-04-25 Published:2004-04-25

Abstract

Abstract: The aUotetraploid citrus somatic hybrid is a new kind of germplasm created via protoplast fusion． We have succeeded in culturing the stems of 9 year-old tree of the somatic hybrid of Cleopatra Mandarin (Citrus reticaulata)and Flying Dragon(Poncirus trifoliata)in vitro，later cryopreserved its shoot tips from in vitro and got regeneration rate by 92% of the cryopreserved shoot-tips．This is the first report on the cryopreservation of citrus somatic hybrid shoot-tips．

Key words: Citrus, Somatic hybrid, Culture in vitro, Cryopreservation

CLC Number:

S666

Wang Zicheng;Deng Xiuxin. Plant Regeneration from the Cryopreserved Somatic Hybrid of Citrus[J]. ACTA HORTICULTURAE SINICA, 2004, 31(2): 215-216.

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Plant Regeneration from the Cryopreserved Somatic Hybrid of Citrus

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