Abstract: Using the stem-segment with single bud as the explant , in vitro culture and high frequency propagation of Chinese wild Vitis including 22 clones of 12 species were studied. The results showed that the most appropriate medium for producing buds to wild Vitis clones was MS +BA 0.5 - 1.0 mg·L ^{- 1} + IBA 0.2 mg·L ^{- 1} except the clone Tangwei and for rooting was 1/ 2 MS + IBA 0.1 - 0.2 mg·L^{- 1} . The optimum midum for subculture and rooting to a few clones was 1/ 2 MS + IBA 0.1 - 0.2 mg·L ^{- 1} + NAA 0.02 mg·L ^{- 1} . In addition , the optimum medium for producing buds and rooting of explants of Baihe-35-1 , Huadong (The clone has not been named) ,Liuba-7 , Zuoshan-1 , Anlin-3 , Yanshan-1 , Guangxi-1 , Guangxi-2 and Taishan-1 was 1/ 2 MS + IBA 0.2 mg·L ^{- 1} , Which could develop seedling directly. As seedling domestication matrixes , pearlite and nutrient soil were adopted and the survival percent of seedlings could reach 90 %.

Key words: Vitis, Chinese wild Vitis, Stem-segment with single bud, In vitro culture, High frequency propagation