Abstract: Using the leaves as explants in vitro culture，it has the advantages of convenient materials，abundant test materials，and low cost. However，the induced regeneration rate of leaves is extremely low，which is a major problem in vitro culture of Hippeastrum vittatum. Effects about different plant growth regulators and different parts of materials on adventitious bud induction and subculture were studied with the leaves of‘Blossom Peacock’and‘Royal Velvet’plantlet. The results showed that the best explants were the base of young leaves（0.5 cm）formed by culturing in MS medium for 10 days. The photoperoid is 16 h · d-1（light intensity 36 μmol · m-2· s-1）. The best medium for adventitious buds induction of Hippeastrum vittatum was MS + 2 mg · L-1 6-BA + 1 mg · L-1 NAA + 2 mg · L-1 TDZ. The adventitious buds of both varieties occurred in an indirect way，and the‘Blossom Peacock’formed callus after 40 days of culture，adventitious buds formed on 55 days，the induction rate reached 69.44%；‘Royal Velvet’formed callus after 45 days of culture，and adventitious buds formed on 65 days，the induction rate reached 66.67%. The optimal adventitious bud proliferation medium was MS + 2 mg · L-1 6-BA + 1 mg · L-1 NAA + 1 mg · L-1 TDZ，and the proliferation coefficients of‘Blossom Peacock’and‘Royal Velvet’reached 4.67 and 3.46，respectively. The optimal somatic embryo induction medium was MS + 2 mg · L-1 6-BA + 1 mg · L-1 PIC，and the induction rate of‘Blossom Peacock’and‘Royal Velvet’reached 66.67% and 63.89%，respectively. Rooting culture was carried out in MS medium without added hormones，and the rooting rate of both varieties reached 100% after 30 days. The Hippeastrum vittatum plantlets after 30 days of rooting culture were moved to room temperature for 3 days. Then remove the sealing film domestication for 3 days. They were transplanted to the high temperature sterilized charcoal︰vermiculite（volume ratio） 1︰1 matrix，and then the survival rate reached 100%.

Key words: Hippeastrum vittatum, in vitro culture, efficient regeneration, plantlet leaves