LEA3 Gene Transferred into Strawberry

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (03): 322-324.

• 研究报告 • Previous Articles Next Articles

LEA₃ Gene Transferred into Strawberry

Wang Junli^1,2; Ge Huibo³;Peng Shiqi³; Zhang Hongmei¹; Xu Jiuru²

( ¹Colege of Science，Hebei University，Baoding 071002，China; ²College of Biological Science and Technology,Beijing Forestry University, 100083, China; ³College of Horticulture，Hebei Agricultural University，Baoding 071001,China)

Received:2002-06-17 Revised:2002-11-04 Online:2003-06-25 Published:2003-06-25

Abstract

Abstract: Callus of anther from strawberry (Fragaria ananassa Duch. Darslect) was induced on MS medium. Late embryogenesis abundant protein gene，LEA3，from barley(Hordeum vulgare L．)was introduced into strawberry callus by the aid of biolistic-mediated transformation method．After 4 times of PPI’selected culture. resistant calli and regenerated plants were obtained．The hybridization bands were determined in diferent transgenic plants by Southem blot，and this showed that LEA₃gene was integrated into strawberry genome.

Key words: Strawberry, Anther, Callus, LEA₃ gene, Transformation

CLC Number:

Wang Junli;Ge Huibo;Peng Shiqi;Zhang Hongmei;Xu Jiuru. LEA₃ Gene Transferred into Strawberry[J]. ACTA HORTICULTURAE SINICA, 2003, 30(03): 322-324.

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LEA₃ Gene Transferred into Strawberry

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