Abstract: Inflorescences were induced by culturing flower-stalk buds of Phalaenopsis on V&W medium containing vitamin component of B5 medium and 5.0 mg·L^-1 benzylaminopurine(BA) under 22°C．Adventitious shoot were induced from the intemodal segments of inflorescence by using the NS medium containing vitamin of B5 medium 0.5 mg·L^-1BA and 0. mg·L^-1 NAA．The low sucrose concentration benefits the shoot's diferentiation and growth． Shoot were propagated on the same medium and developed into plantlet on Hyponex 1 nlediuln containing 100 g·L^-1 banana juice and 2 g·L^-1 active charcoal．

Key words: Phalaenopsis, Inflorescences, Adventitious shoot, In vitro culture