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ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (6): 579-580.

• 研究报告 • Previous Articles     Next Articles

cDNA Library Construction and Identification of Cabbage

Cao Bihao1,2, Lei Jianjun1,2, Song Hongyuan1, Song Ming1, Yang Zhaohui1, Chengbin Xiang3, David. J . Oliver3,and Li Danqiong1   

  1. (1 Department of Horticulture , South2west Agricultural University , Chongqing 400716 , China ; 2 Department of Horticueture , South2China Agricultural University , Guangzhou 510642 , China ; 3 Iowa state university , 50011 America)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-12-25 Published:2002-12-25

Abstract: A cDNA library of cabbage was constructed and an expression plasmid introduced into E. coli XL12Blue was used as cloning vector. The titer of cDNA library is 2 ×108 - 4 ×108 pfu/ mL ; most of the fragments are more than 500bp , the recombinant rate of the library is about 95 %. Probed with resistant gene fragment , phage in situ hybridization , the hybridization signal is clear , which shows this library is good , and the library had been amplified and stored.

Key words: Cabbage, cDNA library

CLC Number: