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ACTA HORTICULTURAE SINICA ›› 2001, Vol. 28 ›› Issue (5): 472-474.

• 研究报告 • Previous Articles     Next Articles

Studies on Anther Culture of Lily

Chu Yunxia1 , Chen Longqing2 , Huang Yanwen1 , and Zhang Yongchun3   

  1. (1 Science department of Huazhong Agricultural University , Wuhan 430070;  2 Forestry department of Huazhong Agricultural University , Wuhan 430070; 3 Horticultural Research Institute , Shanghai Academy of Agricultural Science ,Shanghai 201106)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-10-25 Published:2001-10-25

Abstract: The following experimental results have been obtained : Compared with other stage , microspores at uninucleate stage were more suitable for culture. Calli were induced from anthers of lily placed on modified MS media
supplemented with different concentrations of 2 ,4-dichlorophenoxyacetic acid (2 ,4-D) and Kinetin (KT) according to homogeneous design. The cold pretreatment (4 ℃, 2 days) couldn’t obviously raise the callus induction frequency in anther culture ; The pollen calli were transferred to modified MS media with different concentrations of naphthaleneacetic acid (NAA) . The results indicated that modified MS media with 2. 0 - 4. 0 mg·L-1 NAA were optimal for the differentiation of pollen callus. Cytological examination of mitotic root tip cells from green plants showed that about 25 % of them were haploid and others were diploid.

Key words: Lily, Anther, Tissue culture

CLC Number: