Preliminary Study on Transformation of Tumor Suppressor Gene p53 into Tomato

ACTA HORTICULTURAE SINICA ›› 2001, Vol. 28 ›› Issue (4): 356-358.

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Preliminary Study on Transformation of Tumor Suppressor Gene p53 into Tomato

Li Xiaodong¹, Cao Wanhong¹, Liu Ling¹, Chen Hang¹, and Cheng Zhihui²

( ¹Vegetable Germplasm Impr ovement Laboratory , Beij ing Vegetable Research Center , Beijing 100089; 􀀁 ²Academy of Horticulture , Northwest Sci􀀁Tech University of Agriculture and Forestry , Yangling 712100)

Received:1900-01-01 Revised:1900-01-01 Online:2001-08-25 Published:2001-08-25

Abstract

Abstract: The strain GV3101 of Agrobacterium tumerf aciens harboring a plasmid carrying a human wild􀀁type tumor suppressor gene p 53 driven by the promoter of phaseolin was used to transform cv. Meiwei Cherry of tomato. Leaves of transgenic tomato plants were used for assaying the existence of foreign gene. Results of histochemical assay, PCR amplification and Southern blotting showed that the human wild􀀁type p 53 gene was integrated into the genome of transgenic tomato plants.

Key words: Tomato, Human wild􀀁, type p 53 gene, Genetic transformation

CLC Number:

Li Xiaodong;Cao Wanhong;Liu Ling;Chen Hang;and Cheng Zhihui. Preliminary Study on Transformation of Tumor Suppressor Gene p53 into Tomato[J]. ACTA HORTICULTURAE SINICA, 2001, 28(4): 356-358.

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Preliminary Study on Transformation of Tumor Suppressor Gene p53 into Tomato

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