Establishment of the Tissue Culture System of Sabina chinensis

doi:10.16420/j.issn.0513-353x.2025-0038

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (3): 867-880.doi: 10.16420/j.issn.0513-353x.2025-0038

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Establishment of the Tissue Culture System of Sabina chinensis

WANG Jiali, LIU Huixin, YUE Kangjie, KANG Hongwei, QIAO Ke, TIAN Xuping^*()

College of Forestry，Shanxi Agricultural University，Taigu，Shanxi 030801，China

Received:2025-03-03 Revised:2025-06-30 Online:2026-03-25 Published:2026-03-20
Contact: TIAN Xuping

Abstract

Abstract:

Using stem segments of Sabina chinensis scale leaves and needle leaves as explants，the effects of different disinfection treatments，culture media，anti-browning agents，and plant hormone ratio on stem segment proliferation and rooting were investigated. The results showed that the optimal disinfection time of 0.1% HgCl₂ for scale leaves and needle leaves was 18 and 16 min，and the pollution rate was 8.52% and 2.51%，respectively. Adding 1.0 mg · L^-1 and 2.0 mg · L^-1PVP to the medium was suitable for anti-browning of scale leaves and needle leaves，respectively. The optimal primary culture medium for scale leaves was DCR + 2.0 mg · L^-1 6-BA + 0.5 mg · L^-1 NAA + 1.0 mg · L^-1PVP，with a bud induction rate of 83.27%，while for needle leaves was DCR + 2.0 mg · L^-1 6-BA + 0.1 mg · L^-1NAA + 2.0 mg · L^-1 PVP，with a bud induction rate of 89.35%. The optimal proliferation medium for scale leaves was DCR + 3.0 mg · L^-1 6-BA + 0.5 mg · L^-1 Trans-ZT + 0.5 mg · L^-1NAA + 1.0 mg · L^-1PVP，with a proliferation rate of 88.93%，while for needle leaves，it was DCR + 3.0 mg · L^-1 6-BA + 0.1 mg · L^-1 Trans-ZT + 0.1 mg · L^-1 NAA + 2.0 mg · L^-1PVP，with a proliferation rate of 93.42%. The optimal rooting medium for scale leaves was 1/2MS + 1.0 mg · L^-1 NAA + 1.0 mg · L^-1 IBA + 1.0 mg · L^-1 PVP，with a rooting rate of 73.29%，while for needle leaves，it was DCR + 2.0 mg · L^-1 IBA + 2.0 mg · L^-1 PVP，with a rooting rate of 71.64%. The survival rates of scale leaves and needle leaves plants in the substrate with a volume ratio of peat to vermiculite of 1︰1 was 76% and 60% respectively.

Key words: Sabina chinensis, leaf shape, browning, rooting

WANG Jiali, LIU Huixin, YUE Kangjie, KANG Hongwei, QIAO Ke, TIAN Xuping. Establishment of the Tissue Culture System of Sabina chinensis[J]. Acta Horticulturae Sinica, 2026, 53(3): 867-880.

Figures/Tables 13

References 35

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叶形 Leaf shape	时间/min Time	污染率/% Pollution rate	褐变率/% Browning rate	成活率/% Survival rate
鳞叶 Scale leaf	15	59.32 ± 10.35 a	40.68 ± 1.28 c	0 c
	16	41.33 ± 11.56 ab	58.67 ± 7.41 bc	0 c
	17	21.36 ± 9.79 b	72.25 ± 11.29 bc	6.39 ± 2.57 b
	18	8.52 ± 1.32 c	80.30 ± 11.72 ab	11.18 ± 1.42 a
	19	1.51 ± 0.19 c	98.49 ± 11.89 a	0 c
针叶 Needle leaf	15	21.66 ± 9.12 a	78.34 ± 12.29 b	0 b
	16	2.51 ± 0.31 b	93.80 ± 11.81 ab	3.69 ± 0.74 a
	17	0 b	100.00 ± 0.00 a	0 b
	18	0 b	100.00 ± 0.00 a	0 b
	19	0 b	100.00 ± 0.00 a	0 b

叶形 Leaf shape	时间/min Time	污染率/% Pollution rate	褐变率/% Browning rate	成活率/% Survival rate
鳞叶 Scale leaf	15	59.32 ± 10.35 a	40.68 ± 1.28 c	0 c
	16	41.33 ± 11.56 ab	58.67 ± 7.41 bc	0 c
	17	21.36 ± 9.79 b	72.25 ± 11.29 bc	6.39 ± 2.57 b
	18	8.52 ± 1.32 c	80.30 ± 11.72 ab	11.18 ± 1.42 a
	19	1.51 ± 0.19 c	98.49 ± 11.89 a	0 c
针叶 Needle leaf	15	21.66 ± 9.12 a	78.34 ± 12.29 b	0 b
	16	2.51 ± 0.31 b	93.80 ± 11.81 ab	3.69 ± 0.74 a
	17	0 b	100.00 ± 0.00 a	0 b
	18	0 b	100.00 ± 0.00 a	0 b
	19	0 b	100.00 ± 0.00 a	0 b

叶形 Leaf shape	种类 Type	成活率/% Survival rate	褐变率/% Browning rate
鳞叶 Scale leaf	MS	13.26 ± 1.46 bc	86.74 ± 11.58 ab
	1/2MS	26.35 ± 4.39 b	73.65 ± 10.41 b
	WPM	17.83 ± 2.96 b	82.17 ± 11.22 b
	White	4.85 ± 1.17 c	95.15 ± 9.69 a
	DCR	46.32 ± 4.69 a	53.68 ± 7.51 c
针叶 Needle leaf	MS	4.73 ± 1.22 bc	95.27 ± 10.09 ab
	1/2MS	8.67 ± 2.41 b	91.33 ± 9.77 b
	WPM	2.37 ± 0.85 c	97.63 ± 12.96 a
	White	0 c	100.00 ± 0.00 a
	DCR	60.84 ± 5.93 a	39.16 ± 6.51 c

叶形 Leaf shape	种类 Type	成活率/% Survival rate	褐变率/% Browning rate
鳞叶 Scale leaf	MS	13.26 ± 1.46 bc	86.74 ± 11.58 ab
	1/2MS	26.35 ± 4.39 b	73.65 ± 10.41 b
	WPM	17.83 ± 2.96 b	82.17 ± 11.22 b
	White	4.85 ± 1.17 c	95.15 ± 9.69 a
	DCR	46.32 ± 4.69 a	53.68 ± 7.51 c
针叶 Needle leaf	MS	4.73 ± 1.22 bc	95.27 ± 10.09 ab
	1/2MS	8.67 ± 2.41 b	91.33 ± 9.77 b
	WPM	2.37 ± 0.85 c	97.63 ± 12.96 a
	White	0 c	100.00 ± 0.00 a
	DCR	60.84 ± 5.93 a	39.16 ± 6.51 c

种类 Type	浓度/（mg · L^-1） Concentration	鳞叶Scale leaf		针叶Needle leaf
种类 Type	浓度/（mg · L^-1） Concentration	褐变率/% Browning rate	成活率/% Survival rate	褐变率/% Browning rate	成活率/% Survival rate
对照（Control）	0	51.83 ± 6.27 ab	48.17 ± 5.73 bc	40.19 ± 5.62 b	59.81 ± 7.19 bc
活性炭（AC）	500	48.22 ± 7.54 b	51.78 ± 6.24 b	32.47 ± 4.31 bc	67.83 ± 6.42 b
	1 000	43.89 ± 8.91 b	56.11 ± 8.86 b	30.49 ± 7.91 bc	69.51 ± 7.64 b
	2 000	37.18 ± 9.22 bc	62.82 ± 9.43 b	22.39 ± 9.66 bc	77.61 ± 6.91 b
	3 000	53.86 ± 10.49 ab	46.14 ± 7.81 bc	48.65 ± 7.62 ab	51.35 ± 8.61 bc
聚乙烯吡咯烷酮（PVP）	0.5	51.35 ± 12.23 b	48.65 ± 9.18 bc	19.65 ± 9.13 c	80.35 ± 7.93 ab
	1.0	8.19 ± 1.34 d	91.81 ± 8.66 a	18.21 ± 9.76 c	81.79 ± 6.44 ab
	2.0	17.91 ± 3.91 c	82.09 ± 8.04 ab	6.75 ± 1.13 d	93.25 ± 6.82 a
	3.0	19.83 ± 7.19 c	80.17 ± 11.51 ab	14.26 ± 2.45 c	85.74 ± 7.64 ab
硝酸银（AgNO₃）	0.5	63.41 ± 8.19 a	36.59 ± 6.33 c	44.19 ± 6.73 ab	55.81 ± 8.12 bc
	1.0	66.19 ± 7.33 a	33.81 ± 5.42 c	49.28 ± 7.85 a	50.72 ± 5.19 bc
	2.0	54.18 ± 8.62 ab	45.82 ± 4.39 bc	55.83 ± 6.77 a	44.17 ± 6.93 c
	3.0	42.31 ± 5.44 b	57.69 ± 5.73 b	37.24 ± 7.45 b	62.76 ± 5.91 b

Establishment of the Tissue Culture System of Sabina chinensis

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叶形 Leaf shape	6-BA/ （mg · L^-1）	NAA/ （mg · L^-1）	芽诱导率/% Bud induction rate	株高/cm Plant height	生长状况 Growth status
鳞叶 Scale leaf	0.5	0.1	33.47 ± 6.89 c	2.31 ± 0.12 bc	芽生长慢，叶泛黄，植株细弱 Slow bud growth，yellowing leaves，weak plants
	1.0	0.1	59.63 ± 7.91 b	2.07 ± 0.17 bc	芽生长慢，叶绿色，植株细弱 Slow bud growth，green leaves，weak plants
	2.0	0.1	73.93 ± 7.12 ab	1.84 ± 0.11 c	芽生长快，叶绿色，植株细弱 Fast bud growth，green leaves，weak plants
	0.5	0.5	19.85 ± 4.83 c	2.69 ± 0.26 b	芽生长较快，叶泛黄，植株健壮 Relatively fast bud growth，yellowing leaves，robust plants
	1.0	0.5	49.15 ± 8.53 bc	3.52 ± 0.31 b	芽生长较慢，叶绿色，植株健壮 Relatively slow bud growth，green leaves，robust plants
	2.0	0.5	83.27 ± 9.33 a	4.33 ± 0.29 ab	芽生长快，叶绿色，植株健壮 Fast bud growth，green leaves，robust plants
	0.5	1.0	13.64 ± 2.19 c	5.42 ± 1.03 a	芽生长慢，叶泛黄，植株健壮 Slow bud growth，yellowing leaves，robust plants
	1.0	1.0	28.35 ± 7.63 c	5.03 ± 0.65 a	芽生长较快，叶绿色，植株细弱 Relatively fast bud growth，green leaves，weak plants
	2.0	1.0	46.28 ± 6.77 bc	4.37 ± 0.41 ab	芽生长快，叶泛黄，植株细弱 Fast bud growth，yellowing leaves，weak plants
针叶 Needle leaf	0.5	0.1	49.34 ± 6.19 c	3.59 ± 0.62 bc	芽生长慢，叶泛黄，植株细弱 Slow bud growth，yellowing leaves，weak plants
	1.0	0.1	67.81 ± 5.98 b	5.40 ± 1.03 a	芽生长快，叶绿色，植株健壮 Fast bud growth，green leaves，robust plants
	2.0	0.1	89.35 ± 7.33 a	5.83 ± 1.20 a	芽生长快，叶绿色，植株健壮 Fast bud growth，green leaves，robust plants
	0.5	0.5	64.23 ± 6.34 b	1.35 ± 0.13 c	芽生长较快，叶泛黄，植株健壮 Relatively fast bud growth，yellowing leaves，robust plants
	1.0	0.5	76.18 ± 8.92 ab	2.09 ± 0.20 c	芽生长较快，叶绿色，植株健壮 Relatively fast bud growth，green leaves，robust plants
	2.0	0.5	81.33 ± 7.18 a	3.42 ± 0.83 bc	芽生长快，叶绿色，植株健壮 Fast bud growth，green leaves，robust plants
	0.5	1.0	41.35 ± 5.33 c	5.33 ± 1.09 a	芽生长慢，叶泛黄，植株健壮 Slow bud growth，yellowing leaves，robust plants
	1.0	1.0	61.34 ± 6.81 b	4.83 ± 1.22 b	芽生长慢，叶绿色，植株细弱 Slow bud growth，green leaves，weak plants
	2.0	1.0	78.23 ± 8.49 ab	4.27 ± 1.04 b	芽生长较快，叶绿色，植株细弱 Relatively fast bud growth，green leaves，weak plants

叶形 Leaf shape	6-BA/ （mg · L^-1）	Trans-ZT/ （mg · L^-1）	NAA/（mg · L^-1）	增殖率/% Proliferation rate	增殖系数 Proliferation coefficient	株高 Plant height	生长状况 Growth status
鳞叶 Scale leaf	1.0	0.1	0.05	23.48 ± 4.32 c	+	1.41 ± 0.12 c	叶泛黄，植株细弱，无愈伤组织 Yellowing leaves，weak plants，no callus
	1.0	0.5	0.1	29.83 ± 6.41 c	+	2.05 ± 0.33 c	叶嫩绿，植株细弱，无愈伤组织 Tender green leaves，weak plants，no callus
	1.0	1.0	0.5	56.81 ± 6.03 bc	+	2.74 ± 0.15 bc	叶嫩绿，植株健壮，愈伤组织紧实Tender green leaves，robust plants，compact callus tissue
	2.0	0.1	0.5	49.17 ± 5.41 bc	++	2.83 ± 0.52 b	叶嫩绿，植株健壮，愈伤组织紧实Tender green leaves，robust plants，compact callus tissue
	2.0	0.5	0.05	49.16 ± 5.19 bc	+++	2.96 ± 0.44 b	叶泛黄，植株细弱，愈伤组织疏松Yellowing leaves，weak plants，loose callus tissue
	2.0	1.0	0.1	73.26 ± 9.77 b	+++	2.76 ± 0.17 bc	叶嫩绿，植株健壮，无愈伤组织 Tender green leaves，robust plants，no callus
	3.0	0.1	0.1	81.42 ± 6.55 a	++++	3.07 ± 0.59 b	叶嫩绿，植株健壮，愈伤组织紧实Tender green leaves，robust plants，compact callus tissue
	3.0	0.5	0.5	88.93 ± 8.71 a	++++	4.79 ± 0.41 a	叶嫩绿，植株健壮，愈伤组织紧实Tender green leaves，robust plants，compact callus tissue
	3.0	1.0	0.05	80.13 ± 5.49 a	++++	4.09 ± 0.61 a	叶泛黄，植株健壮，愈伤组织疏松Yellowing leaves，robust plants，loose callus tissue
针叶 Needle	1.0	0.1	0.05	42.37 ± 6.89 c	++	2.34 ± 0.19 c	叶泛黄，植株细弱，无愈伤组织 Yellowing leaves，weak plants，no callus
leaf	1.0	0.5	0.1	55.91 ± 8.23 c	++	3.34 ± 0.33 bc	叶嫩绿，植株健壮，无愈伤组织 Tender green leave，robust plants，no callus
	1.0	1.0	0.5	44.71 ± 7.39 c	++	3.14 ± 0.52 bc	叶泛黄，植株细弱，无愈伤组织 Yellowing leaves，weak plants，no callus
	2.0	0.1	0.5	76.51 ± 9.32 ab	+++	4.34 ± 0.48 b	叶嫩绿，植株健壮，无愈伤组织 Tender green leaves，robust plants，no callus
	2.0	0.5	0.05	70.25 ± 9.88 b	+++	4.96 ± 0.69 ab	叶嫩绿，植株健壮，愈伤组织紧实Tender green leaves，robust plants，compact callus
针叶 Needle	2.0	1.0	0.1	84.22 ± 9.03 a	++++	4.11 ± 0.40 b	叶嫩绿，植株健壮，愈伤组织紧实Tender green leaves，robust plants，compact callus
leaf	3.0	0.1	0.1	93.42 ± 8.71 a	++++	5.17 ± 0.69 a	叶嫩绿，植株健壮，愈伤组织紧实Tender green leaves，robust plants，compact callus
	3.0	0.5	0.5	87.65 ± 7.32 a	++++	5.37 ± 0.82 a	叶嫩绿，植株健壮，愈伤组织疏松 Tender green leaves，robust plants，loose callus
	3.0	1.0	0.05	90.22 ± 8.06 a	++++	5.09 ± 0.92 a	叶泛黄，植株细弱，愈伤组织疏松 Yellowing leaves，weak plants，loose callus

叶形 Leaf shape	种类 Type	NAA/（mg · L^-1）	生根率/% Rooting rate	根长/cm Root length	根数 Root number	株高/cm Plant height
鳞叶 Scale leaf	MS	0.1	0 c	0 c	0 b	1.87 ± 0.26 b
	1/2MS		8.39 ± 1.06 a	1.29 ± 0.23 a	1.03 ± 0.06 a	2.35 ± 0.24 a
	WPM		0 c	0 c	0 b	2.05 ± 0.12 ab
	White		0 c	0 c	0 b	1.43 ± 0.31 c
	DCR		2.45 ± 0.25 b	0.65 ± 0.04 b	1.07 ± 0.05 a	1.57 ± 0.22 bc
针叶 Needle leaf	MS	0.1	0 c	0 c	0 b	2.47 ± 0.15 b
	1/2MS		2.65 ± 0.53 b	1.14 ± 0.22 b	1.26 ± 0.46 ab	2.07 ± 0.33 bc
	WPM		0 c	0 c	0 b	1.74 ± 0.28 bc
	White		0 c	0 c	0 b	1.33 ± 0.11 c
	DCR		9.65 ± 0.14 a	1.41 ± 0.24 a	1.32 ± 0.12 a	2.85 ± 0.27 a
叶形 Leaf shape	种类 Type	NAA/（mg · L^-1）	生长状况 Growth status
鳞叶 Scale leaf	MS	0.1	愈伤组织松散，水渍化，植株细弱，未生根 Loose callus，waterlogging symptoms，weak plants，without root formation
	1/2MS		无愈伤组织，植株健壮，根细短 No callus，robust plants，thin and short roots
	WPM		愈伤组织紧实，有白色斑点，植株健壮，未生根 Compact callus with white spots，robust plants，without root formation
	White		无愈伤组织，植株细弱，未生根 No callus，weak plants，without root formation
	DCR		愈伤组织分化根，植株细弱，根短粗 Roots differentiate from the callus，weak plants，thick and short root
针叶	MS	0.1	愈伤组织疏松，植株健壮，未生根 Loose callus，robust plants，without root formation
Needle leaf	1/2MS		愈伤组织分化根，植株健壮，根细长 Roots differentiate from the callus，robust plants，long and slender roots
	WPM		愈伤组织紧实，植株细弱，未生根 Compact callus，weak plants，without root formation
	White		无愈伤组织，植株细弱，未生根 No callus，weak plants，without root formation
	DCR		无愈伤组织，植株健壮，根长且壮 No callus，robust plants，long and strong roots

NAA/ （mg · L^-1）	IBA/（mg · L^-1）	生根率/% Rooting rate	根长/cm Root length	根数 Root number	株高/cm Plant height
—	—	0 d	0 d	0 d	1.64 ± 0.27 c
0.5	—	13.47 ± 1.09 c	1.84 ± 0.17 bc	2.01 ± 0.25 bc	2.36 ± 0.43 bc
1.0	—	32.19 ± 2.85 bc	2.41 ± 0.38 b	2.66 ± 0.35 b	3.01 ± 0.51 b
2.0	—	19.28 ± 3.07 bc	2.51 ± 0.74 b	1.56 ± 0.23 c	3.32 ± 0.18 b
—	0.5	5.62 ± 0.15 c	0.73 ± 0.08 cd	1.09 ± 0.02 cd	1.63 ± 0.19 c
—	1.0	9.68 ± 0.16 c	1.08 ± 0.21 c	1.65 ± 0.34 c	1.72 ± 0.14 c
—	2.0	8.57 ± 0.61 c	1.23 ± 0.11 c	1.49 ± 0.20 c	2.54 ± 0.29 bc
0.5	0.5	51.93 ± 4.51 b	3.08 ± 0.81 ab	2.87 ± 0.71 b	2.92 ± 0.64 b
1.0	1.0	73.29 ± 5.98 a	3.79 ± 0.93 a	4.23 ± 0.87 a	5.61 ± 0.58 a
2.0	2.0	57.61 ± 5.30 b	3.96 ± 0.72 a	4.06 ± 0.98 a	5.58 ± 0.42 a
NAA/ （mg · L^-1）	IBA/（mg · L^-1）	生长状况 Growth status
—	—	无愈伤组织，植株健壮，未生根 No callus，robust plants，without root formation
0.5	—	愈伤组织分化根，植株健壮，根短粗 Roots differentiate from the callus，robust plant，thick and short roots
1.0	—	无愈伤组织，植株健壮，根细长 No callus，robust plants，slender and long roots
2.0	—	无愈伤组织，植株细弱，根细长 No callus，weak plants，slender and long roots
—	0.5	愈伤组织分化根，植株细弱，根短粗Roots differentiate from the callus，weak plants，thick and short roots
—	1.0	愈伤组织分化根，植株健壮，根短粗Roots differentiate from the callus，robust plants，thick and short roots
—	2.0	无愈伤组织，植株健壮，根细长 No callus，robust plants，slender and long roots
0.5	0.5	无愈伤组织，植株健壮，根长且壮 No callus，robust plants，long and strong roots
1.0	1.0	无愈伤组织，植株健壮，根长且壮 No callus，robust plants，long and strong roots
2.0	2.0	无愈伤组织，植株健壮，根细长 No callus，robust plants，slender and long roots

NAA/（mg · L^-1）	IBA/（mg · L^-1）	生根率/% Rooting rate	根长/cm Root length	根数 Root number	株高/cm Plant height
—	—	0 d	0 d	0 d	1.64 ± 0.27 c
0.5	—	16.72 ± 1.04 c	1.14 ± 0.13 c	1.41 ± 0.27 c	1.77 ± 0.41 c
1.0	—	50.84 ± 5.49 bc	2.87 ± 0.28 c	1.49 ± 0.25 c	2.07 ± 0.56 bc
2.0	—	70.93 ± 6.82 a	3.29 ± 0.44 bc	2.04 ± 0.13 b	2.87 ± 0.37 b
—	0.5	15.77 ± 2.05 c	1.92 ± 0.21 c	1.19 ± 0.20 c	1.76 ± 0.13 c
—	1.0	61.73 ± 3.97 b	3.49 ± 0.71 bc	1.88 ± 0.44 b	2.76 ± 0.59 b
—	2.0	71.64 ± 7.81 a	4.95 ± 0.94 a	2.67 ± 0.16 a	4.32 ± 0.75 a
0.5	0.5	55.72 ± 4.97 bc	4.10 ± 0.29 b	1.40 ± 0.23 c	1.46 ± 0.67 c
1.0	1.0	62.14 ± 6.81 b	4.56 ± 0.86 ab	2.20 ± 0.52 ab	2.69 ± 0.38 b
2.0	2.0	45.17 ± 7.78 bc	2.97 ± 0.35 c	1.83 ± 0.29 b	1.93 ± 0.40 c
NAA/（mg · L^-1）	IBA/（mg · L^-1）	生长状况 Growth status
—	—	无愈伤组织，植株健壮，未生根 No callus，robust plants，without root formation
0.5	—	愈伤组织分化根，植株细弱，根短粗 Roots differentiate from the callus，weak plants，thick and short roots
1.0	—	愈伤组织分化根，植株健壮，根短粗 Roots differentiate from the callus，robust plants，thick and short roots
2.0	—	愈伤组织分化根，植株细弱，根长且壮 Roots differentiate from the callus，weak plants，long and strong roots
—	0.5	愈伤组织分化根，植株细弱，根短粗 Roots differentiate from the callus，weak plants，thick and short roots
—	1.0	无愈伤组织，植株健壮，根细长 No callus，robust plants，slender and long roots
—	2.0	无愈伤组织，植株健壮，根长且壮 No callus，robust plants，long and strong roots
0.5	0.5	愈伤组织分化根，植株健壮，根短粗 Roots differentiate from the callus，robust plants，thick and short roots
1.0	1.0	无愈伤组织，植株健壮，根细长 No callus，robust plants，slender and long roots
2.0	2.0	无愈伤组织，植株细弱，根细长 No callus，weak plants，slender and long roots

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[5]	SU Yanli, GAO Xiaoming, YANG Jian, WANG Long, WANG Suke, ZHANG Xiangzhan, XUE Huabai. Dynamic Changes of Browning Degree，Phenolics Contents and Related Enzyme Activities During Pear Fruit Development [J]. Acta Horticulturae Sinica, 2022, 49(11): 2304-2312.
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[8]	CHEN Chen, LIU Chenghui, SHI Lijia, JIANG Aili, HU Wenzhong. An Analysis of Alternative Splicing Events During Browning Inhibition of Fresh-cut Apples by Hydrogen Sulfide Treatment [J]. Acta Horticulturae Sinica, 2021, 48(11): 2121-2132.
[9]	WANG Zhihua, JIA Chaoshuang, WANG Wenhui, TONG Wei, and JIANG Yunbin. Effects of Low Temperature Storage on Energy Metabolism，Related Physiology and Quality in‘Jinhong’Apple Fruit [J]. Acta Horticulturae Sinica, 2020, 47(12): 2277-2289.
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