Development and Implementation of an Efficient Method for Evaluating Tomato Resistance to Bacterial Wilt Based on Leaf Injection

doi:10.16420/j.issn.0513-353x.2024-1061

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (4): 1215-1225.doi: 10.16420/j.issn.0513-353x.2024-1061

• New technologies and methods • Previous Articles Next Articles

Development and Implementation of an Efficient Method for Evaluating Tomato Resistance to Bacterial Wilt Based on Leaf Injection

HUANG Xinran¹, WANG Rongbo¹, ZHANG Qianrong², LI Benjin¹, WENG Qiyong¹, LIU Peiqing¹^,^*()

¹ Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests，Institute of Plant Protection，Fujian Academy of Agricultural Sciences，Fuzhou 350013，China
² Crops Research Institute，Fujian Academy of Agricultural Sciences，Fuzhou 350013，China

Received:2025-09-15 Revised:2025-12-18 Online:2026-04-25 Published:2026-04-20
Contact: LIU Peiqing

Abstract

Abstract:

Bacterial wilt，caused by Ralstonia solanacearum，is a significant crop disease worldwide，particularly in southern China. The adoption of resistant varieties remains the most economical and effective strategy for managing this soil-borne disease. Identification and screening of bacterial wilt-resistant germplasms are fundamental to crop disease resistance breeding. In this study，a leaf-injection inoculation method was established for the identification of bacterial wilt-resistant germplasms of tomato，while five highly virulent R. solanacearum strains with varied pathogenicity，collected from Fujian province were combined and utilized as an identification strain mix. Three germplasms，rootstock 6，8 and 10，were identified as resistant in the screening. Further，the leaf injection method was compared for its effectiveness with the traditional root injuring and root soaking inoculation methods. While the leaf injection method could be simply operated，results showed that it required less inocula and shorter experimental time to provide more clear and consistent symptoms than the traditional methods. In addition，this method was further evaluated and validated for its effectiveness on other crops in Solanaceae.

Key words: tomato, bacterial wilt of leaf injection, identification of resistance, Ralstonia solanacearum

HUANG Xinran, WANG Rongbo, ZHANG Qianrong, LI Benjin, WENG Qiyong, LIU Peiqing. Development and Implementation of an Efficient Method for Evaluating Tomato Resistance to Bacterial Wilt Based on Leaf Injection[J]. Acta Horticulturae Sinica, 2026, 53(4): 1215-1225.

Figures/Tables 8

References 25

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基因名称Gene name	上游引物序列（5′-3′）Forward primer	下游引物序列（5′-3′）Reverse primer
16S rRNA	GTCGCCGTCAACTCACTTTCC	GTCGCCGTCAGCAATGCGGAATCG
SlEF1a	ACCTTTGCTGAATACCCTCCATTG	CACAGTTCACTTCCCCTTCTTCTG

基因名称Gene name	上游引物序列（5′-3′）Forward primer	下游引物序列（5′-3′）Reverse primer
16S rRNA	GTCGCCGTCAACTCACTTTCC	GTCGCCGTCAGCAATGCGGAATCG
SlEF1a	ACCTTTGCTGAATACCCTCCATTG	CACAGTTCACTTCCCCTTCTTCTG

名称Name	材料Material	叶片注射法Leaf injection inoculation		伤根接种法Root injury inoculation
名称Name	材料Material	病情指数DI	表型Phenotype	病情指数DI	表型Phenotype
感病对照品种 Susceptible control varieties	AC	70.37	S	60.42	S
感病对照品种 Susceptible control varieties	千禧Cherry tomato	68.52	S	56.25	S
抗病对照品种 Disease-resistant control varieties	桂砧1号Guizhen 1	12.90	R	12.50	R
待测砧木材料 Rootstock material to be tested	番茄砧木6号Tomato rootstock 6	61.11	S	54.16	S
	番茄砧木405 Tomato rootstock 405	69.44	S	56.25	S
	浙砧1号Zhezhen 1	70.37	S	60.42	S
	砧1 Zhen 1	37.04	MR	22.92	R
	砧2 Zhen 2	70.37	S	58.33	S
	砧3 Zhen 3	41.66	MR	20.83	R
	砧4 Zhen 4	37.04	MR	31.25	MR
	砧5 Zhen 5	44.44	MR	35.41	MR
	砧6 Zhen 6	18.52	R	12.50	R
	砧7 Zhen 7	33.33	MR	22.92	R
	砧8 Zhen 8	20.37	R	16.67	R
	砧9 Zhen 9	25.92	MR	20.83	R
	砧10 Zhen 10	22.22	R	18.75	R

名称Name	材料Material	叶片注射法Leaf injection inoculation		伤根接种法Root injury inoculation
名称Name	材料Material	病情指数DI	表型Phenotype	病情指数DI	表型Phenotype
感病对照品种 Susceptible control varieties	AC	70.37	S	60.42	S
感病对照品种 Susceptible control varieties	千禧Cherry tomato	68.52	S	56.25	S
抗病对照品种 Disease-resistant control varieties	桂砧1号Guizhen 1	12.90	R	12.50	R
待测砧木材料 Rootstock material to be tested	番茄砧木6号Tomato rootstock 6	61.11	S	54.16	S
	番茄砧木405 Tomato rootstock 405	69.44	S	56.25	S
	浙砧1号Zhezhen 1	70.37	S	60.42	S
	砧1 Zhen 1	37.04	MR	22.92	R
	砧2 Zhen 2	70.37	S	58.33	S
	砧3 Zhen 3	41.66	MR	20.83	R
	砧4 Zhen 4	37.04	MR	31.25	MR
	砧5 Zhen 5	44.44	MR	35.41	MR
	砧6 Zhen 6	18.52	R	12.50	R
	砧7 Zhen 7	33.33	MR	22.92	R
	砧8 Zhen 8	20.37	R	16.67	R
	砧9 Zhen 9	25.92	MR	20.83	R
	砧10 Zhen 10	22.22	R	18.75	R

Development and Implementation of an Efficient Method for Evaluating Tomato Resistance to Bacterial Wilt Based on Leaf Injection

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