Effects of Peat Soil and Mushroom Residue Application on the Potato Root-Associated Microbiome and Yield

doi:10.16420/j.issn.0513-353x.2023-0061

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (9): 2143-2154.doi: 10.16420/j.issn.0513-353x.2023-0061

• Cultivation · Physiology & Biochemistry • Previous Articles Next Articles

Effects of Peat Soil and Mushroom Residue Application on the Potato Root-Associated Microbiome and Yield

ZHONG Yang¹, QIN Yazhi¹, LUO Shuai¹, JING Yuling¹, WANG Wanxing², LI Guangcun², HU Xinxi³, QIN Yuzhi³, CHENG Xu¹^,^*(), XIONG Xingyao¹^,^*()

¹ Agricultural Genomics Institute at Shenzhen，Chinese Academy of Agricultural Sciences，Shenzhen，Guangdong 518116，China
² Institute of Vegetable and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China
³ College of Horticulture，Hunan Agricultural University，Changsha 410128，China

Received:2024-05-27 Revised:2024-07-22 Online:2024-09-25 Published:2024-09-19
Contact: CHENG Xu, XIONG Xingyao

Abstract

Abstract:

Improving soil structure of the paddy-upland rotation is an important measure to enhance the efficiency of soil nutrient supply for winter potatoes. In this study，an investigation was undertaken to explore the comprehensive effects of peat soil（PS）and mushroom residue（MR）short-term application on the soil physicochemical properties and nutrient composition of paddy soils，using the potato cultivar ‘Favorita’as the experimental material. High-throughput amplicon sequencing was utilized to clarify the impact of soil improvement treatments on the structure and function of the root-associated microbiome. Results showed that PS and MR treatments have altered the proportionate distribution of soil aggregates to a certain extent；both treatments also significantly increased soil organic matter content，as well as the levels of available phosphorus and potassium. The soil improvement treatments also enhanced the diversity of the rhizosphere microbial community，with Proteobacteria，Actinobacteria，Bacteroidetes，and Firmicutes being the dominant phyla in both rhizosphere and endophytic regions. Principal coordinate analysis（PCoA）results showed that，compared to bacterial communities，fungal community compositions were relatively stable，and less impact on changes induced by soil amendments. Co-occurrence network analysis revealed that PS treatment resulted in tighter node connections and a more prominent network modularity tendency，especially among the endophytic compartment. Canonical Correspondence Analysis （CCA）indicated that soil organic carbon（TOC）and total nitrogen（TN）significantly influenced the composition of both bacterial and fungal communities. Considering the potato yield results under the combined soil improvement treatments，peat soil amendment appears to be more suitable for the amelioration of paddy field soils and has potential for application.

Key words: potato, peat soil, mushroom residue, microbial community, co-occurrence network

ZHONG Yang, QIN Yazhi, LUO Shuai, JING Yuling, WANG Wanxing, LI Guangcun, HU Xinxi, QIN Yuzhi, CHENG Xu, XIONG Xingyao. Effects of Peat Soil and Mushroom Residue Application on the Potato Root-Associated Microbiome and Yield[J]. Acta Horticulturae Sinica, 2024, 51(9): 2143-2154.

Figures/Tables 8

Table 1 Content distribution of soil aggregates under different treatments

处理 Treatment	大团聚体 Macro-aggregates			微团聚体 Micro-aggregates
处理 Treatment	> 2.0 mm	0.25 ~ 2.00 mm	合计Total	0.053 ~ 0.250 mm	< 0.053 mm	合计Total
对照 Control	20.56 ± 1.37	60.22 ± 3.10	80.78 ± 4.34	16.90 ± 3.16	2.32 ± 1.24	19.22 ± 4.34
泥炭土 Peat soil	20.40 ± 0.82	49.14 ± 1.48^*	69.54 ± 2.29^*	24.00 ± 1.94	6.47 ± 0.20^**	30.46 ± 2.12^*
蘑菇渣 Mushroom residue	56.65 ± 3.49^**	33.20 ± 2.40^**	89.86 ± 5.77	8.77 ± 2.16^*	1.37 ± 0.33	10.14 ± 2.48

Table 2 Soil physical and chemical properties under different treatments

处理 Treatment	pH	有机碳/（g · kg^-1） Organic C	全氮/（g · kg^-1） Total N	速效磷/（mg · kg^-1） Available P	速效钾/（mg · kg^-1） Available K	碳氮比 C/N
对照 Control	6.78 ± 0.06	9.78 ± 1.31	0.92 ± 0.11	96.69 ± 8.02	52.00 ± 5.05	10.58 ± 0.19
泥炭土 Peat soil	6.41 ± 0.04^**	89.43 ± 0.75^**	5.38 ± 0.02^**	67.66 ± 10.12^**	187.80 ± 20.16^**	16.63 ± 0.08^**
蘑菇渣Mushroom residue	7.19 ± 0.13^*	53.48 ± 5.15^**	4.93 ± 0.42^**	589.93 ± 3.06^**	1 111.77 ± 79.55^**	10.84 ± 0.13

Fig. 1 Shannon index of bacterial and fungal communities in different potato niches and soil improvement treatments CK：Control；PS：Peat soil；MR：Mushroom residue；EC：Endophytic compartment；RH：Rhizosphere soil；BS：Bulk soil. The same below.

Fig. 2 Relative abundance of bacterial and fungal communities in different potato niches and soil improvement treatments A：Bacterial phylum-level；B：Fungal phylum-level；C：Fungal genus-level.

Fig. 3 PCoA of bacterial and fungal community in different potato niches and soil improvement treatments

Fig. 4 Co-occurrence network analysis of microbial communities in different potato niches and soil improvement treatments A：Co-occurrence network based on the ASV level in all treatments；B-D：Distribution statistics of nodes，edges and average degree of bacterial and fungal communities.

Fig. 5 CCA correlation analysis of environmental factors and different microbial communities in different potato niches and soil improvement treatments MA：Soil aggregate；TOC：Organic C；TN：Total；AP：Available P；AK：Available K.

Fig. 6 The effects of soil improvement treatments on the yield of potato per plant and commercial potatoes rate Statistical differences between treatment and control groups were analysed using a Student’s t-test. * indicates significance at P < 0.05，**** indicates highly significance at P < 0.0001.

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Effects of Peat Soil and Mushroom Residue Application on the Potato Root-Associated Microbiome and Yield

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