Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (11): 2365-2375.doi: 10.16420/j.issn.0513-353x.2022-0985
• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles
ZHANG Yongqi1,2, WANG Chao1,2, XU Linlin2, WU E’jiao2, LI Tianhong1, ZHAO Mizhen2, YUAN Huazhao2,*()
Received:
2023-03-15
Revised:
2023-08-28
Online:
2023-11-25
Published:
2023-11-28
Contact:
YUAN Huazhao
ZHANG Yongqi, WANG Chao, XU Linlin, WU E’jiao, LI Tianhong, ZHAO Mizhen, YUAN Huazhao. Development of SSR Molecular Markers and Construction of Core Collection of Wild Diploid Strawberry[J]. Acta Horticulturae Sinica, 2023, 50(11): 2365-2375.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2022-0985
位点 Loci | 上游保守序列(5′-3′) Upstream conservative sequence | 下游保守序列(5′-3′) Downstream conservative sequence |
---|---|---|
SSR1 | TGCTAAGTAGAAGCCGCCA | AAAGCCATTTTACTACCTAGCC |
SSR4 | TTAACAAGCACACGACAAAG | CATGGGTCTTGCTTATAGCC |
SSR6 | TCCTCCATTATCTGCAGAAATC | TTCCAACCTCAAGCGTCT |
SSR7 | CAAAGAGGGTTTCATTCATG | CGAACCCATGCACAC |
SSR9 | AGACTTGTCTGGCCATTGTA | CACAGTACATCTCGCAAGGC |
SSR10 | GTGACTTGATGTTGCTGTAAGT | CATACTACTGGAGATGCTC |
SSR11 | GGTAATTTTCTTAAGTGACGCC | ATCGAACCAGCAGGCCT |
SSR13 | CACCAATTTCACCACATATC | TTCTGTGAGTCTTGAATGTTGC |
SSR17 | CCTCTCTTGTCCGGAACTT | CAGCAACTCCAAAGTTAAA |
SSR18 | ATGCAATGTGTGTAAACACA | CACGTGCGTTTGTCAAC |
SSR19 | TGTGCATTACTTGCTAATCC | CATCCTTTTGCTTTTGACCA |
SSR20 | AGGCCCATTTCACTTCTGTC | TCACCCAACCCGGAAA |
SSR21 | TATCTAGTAGTTGGAAAAGGA | TGTGGCATGGAAGTGG |
SSR22 | TGCATCATGCATTCAAT | GGAAGCTGCATTTCCATAGAA |
SSR23 | AATGTATATGTAGAATCC | GTCAATTGCCCATAGGTTAGC |
SSR24 | TGATCGAGTTCAATTGAGTCAC | GGATCGAGGACCTAATCCC |
SSR25 | CCCACACAAATTTAAAAGAGA | AGACTGAAATGGCCATGG |
SSR26 | GATTGCTCAAAGTGCTTTAC | GCCTATAATTGCTGTCATGCTAG |
Table 1 Eighteen pairs of conserved sequence information before and after polymorphic SSR
位点 Loci | 上游保守序列(5′-3′) Upstream conservative sequence | 下游保守序列(5′-3′) Downstream conservative sequence |
---|---|---|
SSR1 | TGCTAAGTAGAAGCCGCCA | AAAGCCATTTTACTACCTAGCC |
SSR4 | TTAACAAGCACACGACAAAG | CATGGGTCTTGCTTATAGCC |
SSR6 | TCCTCCATTATCTGCAGAAATC | TTCCAACCTCAAGCGTCT |
SSR7 | CAAAGAGGGTTTCATTCATG | CGAACCCATGCACAC |
SSR9 | AGACTTGTCTGGCCATTGTA | CACAGTACATCTCGCAAGGC |
SSR10 | GTGACTTGATGTTGCTGTAAGT | CATACTACTGGAGATGCTC |
SSR11 | GGTAATTTTCTTAAGTGACGCC | ATCGAACCAGCAGGCCT |
SSR13 | CACCAATTTCACCACATATC | TTCTGTGAGTCTTGAATGTTGC |
SSR17 | CCTCTCTTGTCCGGAACTT | CAGCAACTCCAAAGTTAAA |
SSR18 | ATGCAATGTGTGTAAACACA | CACGTGCGTTTGTCAAC |
SSR19 | TGTGCATTACTTGCTAATCC | CATCCTTTTGCTTTTGACCA |
SSR20 | AGGCCCATTTCACTTCTGTC | TCACCCAACCCGGAAA |
SSR21 | TATCTAGTAGTTGGAAAAGGA | TGTGGCATGGAAGTGG |
SSR22 | TGCATCATGCATTCAAT | GGAAGCTGCATTTCCATAGAA |
SSR23 | AATGTATATGTAGAATCC | GTCAATTGCCCATAGGTTAGC |
SSR24 | TGATCGAGTTCAATTGAGTCAC | GGATCGAGGACCTAATCCC |
SSR25 | CCCACACAAATTTAAAAGAGA | AGACTGAAATGGCCATGG |
SSR26 | GATTGCTCAAAGTGCTTTAC | GCCTATAATTGCTGTCATGCTAG |
位点Loci | Na | Ne | I | Ho | He | PIC |
---|---|---|---|---|---|---|
SSR1 | 27.000 | 9.435 | 2.678 | 0.188 | 0.894 | 0.887 |
SSR4 | 13.000 | 1.479 | 0.836 | 0.006 | 0.324 | 0.315 |
SSR6 | 27.000 | 8.005 | 2.593 | 0.443 | 0.875 | 0.867 |
SSR7 | 24.000 | 5.728 | 2.378 | 0.188 | 0.825 | 0.816 |
SSR9 | 36.000 | 13.120 | 2.981 | 0.216 | 0.924 | 0.920 |
SSR10 | 29.000 | 10.574 | 2.704 | 0.352 | 0.905 | 0.898 |
SSR11 | 16.000 | 1.751 | 1.129 | 0.028 | 0.429 | 0.419 |
SSR13 | 7.000 | 1.687 | 0.911 | 0.051 | 0.407 | 0.387 |
SSR17 | 34.000 | 15.419 | 3.000 | 0.477 | 0.935 | 0.932 |
SSR18 | 18.000 | 2.448 | 1.472 | 0.119 | 0.591 | 0.569 |
SSR19 | 11.000 | 2.886 | 1.560 | 0.119 | 0.653 | 0.632 |
SSR20 | 49.000 | 21.158 | 3.362 | 0.432 | 0.953 | 0.951 |
SSR21 | 23.000 | 8.990 | 2.526 | 0.153 | 0.889 | 0.879 |
SSR22 | 21.000 | 4.063 | 2.015 | 0.222 | 0.754 | 0.739 |
SSR23 | 25.000 | 7.088 | 2.398 | 0.222 | 0.859 | 0.846 |
SSR24 | 30.000 | 14.681 | 2.934 | 0.307 | 0.932 | 0.928 |
SSR25 | 34.000 | 9.835 | 2.746 | 0.347 | 0.898 | 0.891 |
SSR26 | 20.000 | 3.827 | 2.003 | 0.199 | 0.739 | 0.726 |
平均Mean | 24.667 | 7.899 | 2.235 | 0.226 | 0.766 | 0.756 |
Table 2 Genetic diversity of 18 SSR in 176 strawberry materials
位点Loci | Na | Ne | I | Ho | He | PIC |
---|---|---|---|---|---|---|
SSR1 | 27.000 | 9.435 | 2.678 | 0.188 | 0.894 | 0.887 |
SSR4 | 13.000 | 1.479 | 0.836 | 0.006 | 0.324 | 0.315 |
SSR6 | 27.000 | 8.005 | 2.593 | 0.443 | 0.875 | 0.867 |
SSR7 | 24.000 | 5.728 | 2.378 | 0.188 | 0.825 | 0.816 |
SSR9 | 36.000 | 13.120 | 2.981 | 0.216 | 0.924 | 0.920 |
SSR10 | 29.000 | 10.574 | 2.704 | 0.352 | 0.905 | 0.898 |
SSR11 | 16.000 | 1.751 | 1.129 | 0.028 | 0.429 | 0.419 |
SSR13 | 7.000 | 1.687 | 0.911 | 0.051 | 0.407 | 0.387 |
SSR17 | 34.000 | 15.419 | 3.000 | 0.477 | 0.935 | 0.932 |
SSR18 | 18.000 | 2.448 | 1.472 | 0.119 | 0.591 | 0.569 |
SSR19 | 11.000 | 2.886 | 1.560 | 0.119 | 0.653 | 0.632 |
SSR20 | 49.000 | 21.158 | 3.362 | 0.432 | 0.953 | 0.951 |
SSR21 | 23.000 | 8.990 | 2.526 | 0.153 | 0.889 | 0.879 |
SSR22 | 21.000 | 4.063 | 2.015 | 0.222 | 0.754 | 0.739 |
SSR23 | 25.000 | 7.088 | 2.398 | 0.222 | 0.859 | 0.846 |
SSR24 | 30.000 | 14.681 | 2.934 | 0.307 | 0.932 | 0.928 |
SSR25 | 34.000 | 9.835 | 2.746 | 0.347 | 0.898 | 0.891 |
SSR26 | 20.000 | 3.827 | 2.003 | 0.199 | 0.739 | 0.726 |
平均Mean | 24.667 | 7.899 | 2.235 | 0.226 | 0.766 | 0.756 |
Fig. 4 Relationship between the prime number of core species and the proportion of retained alleles extracted from strawberry resources by Core Finder
类型 Type | 种质数Number of germplasms | N | Na | Ne | I | Ho | He | PIC |
---|---|---|---|---|---|---|---|---|
原始种质 Original collection | 176.000 | 444 | 24.667 | 7.899 | 2.235 | 0.226 | 0.766 | 0.756 |
核心种质 Core collection | 43.000 (24.43%) | 366 (82.43%) | 20.333 (82.43%) | 10.497 (132.89%) | 2.498 (111.77%) | 0.260 (115.04%) | 0.852 (111.23%) | 0.842 (111.40%) |
保留种质 Reservation collection | 133.000 (75.57%) | 370 (83.33%) | 20.556 (83.33%) | 6.804 (86.14%) | 2.043 (91.41%) | 0.216 (95.58%) | 0.724 (94.52%) | 0.713 (84.65%) |
t1 | 0.142 | 0.177 | 0.255 | 0.494 | 0.125 | 0.349 | ||
t2 | 0.210 | 0.545 | 0.469 | 0.827 | 0.572 | 0.565 |
Table 3 Comparison of genetic diversity among wild strawberry core collection,original collection and reserved collection
类型 Type | 种质数Number of germplasms | N | Na | Ne | I | Ho | He | PIC |
---|---|---|---|---|---|---|---|---|
原始种质 Original collection | 176.000 | 444 | 24.667 | 7.899 | 2.235 | 0.226 | 0.766 | 0.756 |
核心种质 Core collection | 43.000 (24.43%) | 366 (82.43%) | 20.333 (82.43%) | 10.497 (132.89%) | 2.498 (111.77%) | 0.260 (115.04%) | 0.852 (111.23%) | 0.842 (111.40%) |
保留种质 Reservation collection | 133.000 (75.57%) | 370 (83.33%) | 20.556 (83.33%) | 6.804 (86.14%) | 2.043 (91.41%) | 0.216 (95.58%) | 0.724 (94.52%) | 0.713 (84.65%) |
t1 | 0.142 | 0.177 | 0.255 | 0.494 | 0.125 | 0.349 | ||
t2 | 0.210 | 0.545 | 0.469 | 0.827 | 0.572 | 0.565 |
[1] |
|
[2] |
doi: 10.16420/j.issn.0513-353x.2021-0312 URL |
陈明堃, 陈璐, 孙维红, 马山虎, 兰思仁, 彭东辉, 刘仲健, 艾叶. 2022. 建兰种质资源遗传多样性分析及核心种质构建. 园艺学报, 49 (1):175-186.
doi: 10.16420/j.issn.0513-353x.2021-0312 URL |
|
[3] |
doi: 10.1007/s001220051343 URL |
[4] |
|
崔竣杰, 程蛟文, 曹毅, 胡开林. 2022. 基于SSR标记和表型性状构建苦瓜核心种质的研究. 中国蔬菜,(2):25-32.
|
|
[5] |
doi: 10.1016/j.hpj.2019.11.004 |
[6] |
|
[7] |
|
贺润丽, 韩毅丽, 段静静, 刘计权, 王莉花. 2019. 款冬转录组SSR标记开发及其多态性研究. 中草药, 50 (20):5026-5032.
|
|
[8] |
|
黄小凤, 韦阳连, 袁叶, 余金昌, 袁志永, 王伟山. 2022. 基于SNP分子标记的221份荔枝品种(品系)的遗传多样性分析及核心种质库构建. 植物资源与环境学报, 31 (4):74-84.
|
|
[9] |
doi: 10.16420/j.issn.0513-353x.2020-0497 URL |
蒋爽, 张学英, 安海山, 徐芳杰, 章加应. 2021. 枇杷全基因组SSR标记开发及其多态性研究. 园艺学报, 48 (5):1013-1022.
doi: 10.16420/j.issn.0513-353x.2020-0497 URL |
|
[10] |
doi: 10.16420/j.issn.0513-353x.2020-0608 URL |
江锡兵, 章平生, 徐阳, 吴聪连, 张东北, 龚榜初, 吴开云, 赖俊声. 2021. 栗杂交F1代SSR标记遗传多样性分析. 园艺学报, 48 (5):897-907.
doi: 10.16420/j.issn.0513-353x.2020-0608 URL |
|
[11] |
doi: 10.1016/j.hpj.2021.05.001 URL |
[12] |
doi: 10.1007/s11105-015-0966-7 URL |
[13] |
|
雷家军, 薛莉, 代汉萍, 邓明琴. 2015. 世界草莓属(Fragaria)植物的种类与分布//草莓研究进展(Ⅳ). 北京: 中国农业出版社:364-375.
|
|
[14] |
|
刘艳晶, 孙贵连, 周琴, 邹雪梅, 黄小龙. 2022. 森林草莓FvLysM基因家族的生物信息学分析. 分子植物育种, 20 (9):2864-2875.
|
|
[15] |
|
刘遵春, 刘大亮, 崔美, 李敏, 焦其庆, 高利平, 陈学森. 2012. 整合农艺性状和分子标记数据构建新疆野苹果核心种质. 园艺学报, 39 (6):1045-1054.
|
|
[16] |
|
[17] |
|
缪黎明, 王神云, 邹明华, 李建斌, 孔李俊, 余小林. 2016. 园艺作物核心种质构建的研究进展. 植物遗传资源学报, 17 (5):791-800.
doi: 10.13430/j.cnki.jpgr.2016.05.001 |
|
[18] |
doi: 10.1007/s10528-012-9509-1 URL |
[19] |
|
覃阳, 全绍文, 周丽, 杨洁萍, 牛建新. 2019. 基于转录组测序的核桃(Juglans regia L.)SSR标记开发. 分子植物育种, 17 (20):6736-6742.
|
|
[20] |
|
沈志军, 马瑞娟, 俞明亮, 蔡志翔, 许建兰. 2013. 国家果树种质南京桃资源圃初级核心种质构建. 园艺学报, 40 (1):125-134.
|
|
[21] |
doi: 10.1038/ng.740 |
[22] |
|
宋琳琳, 潘佳颖, 李梦思, 周晓君. 2022. 大叶铁线莲(Clematis heracleifolia DC.)多态性SSR引物开发. 分子植物育种, 20 (2):448-454.
|
|
[23] |
|
孙永强, 陈建华, 张剑, 董胜君, 刘权钢, 刘青柏. 2022. 基于表型性状的西伯利亚杏核心种质构建. 沈阳农业大学学报, 53 (1):43-54.
|
|
[24] |
doi: 10.1007/s001220051342 URL |
[25] |
|
汪磊, 王姣梅, 汪魏, 王玲, 王力军, 严兴初, 谭美莲. 2021. 基于表型多样性构建向日葵核心种质. 中国油料作物学报, 43 (6):1052-1060.
doi: 10.19802/j.issn.1007-9084.2020255 |
|
[26] |
|
曾钦朦, 曾亚军, 陈胜群, 侯娜. 2022. 贵州核桃核心种质筛选与构建研究. 西北植物学报, 42 (5):865-873.
|
|
[27] |
|
张春雨, 陈学森, 张艳敏, 苑兆和, 刘遵春, 王延龄, 林群. 2009. 采用分子标记构建新疆野苹果核心种质的方法. 中国农业科学, 42 (2):597-604.
|
|
[28] |
|
张利达, 唐克轩. 2010. 植物EST-SSR标记开发及其应用. 基因组学与应用生物学, 29 (3):534-541.
|
|
[29] |
|
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