Identification and Expression Profiling of Aux/IAA Family Gene During Peach Fruit Ripening

doi:10.16420/j.issn.0513-353x.2016-0388

ACTA HORTICULTURAE SINICA ›› 2017, Vol. 44 ›› Issue (2): 233-244.doi: 10.16420/j.issn.0513-353x.2016-0388

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Identification and Expression Profiling of Aux/IAA Family Gene During Peach Fruit Ripening

ZENG Wenfang，WANG Xiaobei，PAN Lei，NIU Liang，LU Zhenhua，CUI Guochao，and WANG Zhiqiang^*

Zhengzhou Fruit Research Institute，Chinese Academy of Agricultural Sciences，National Peach and Grape Improvement Center，Key Laboratory of Fruit Breeding Technology of Ministry of Agriculture，Zhengzhou 450009，China

Online:2017-02-25 Published:2017-02-25

Abstract

Abstract:

Auxin/indole-3-acetic acid（Aux/IAA）is involved in plant growth，development，and multiple other processes. In this study，the Aux/IAA gene family in the peach genome was identified and analyzed by bioinformatics，additionally，the expression levels of Aux/IAA genes were detected by qRT-PCR in melting-flesh and stony-hard peach fruits during fruit ripening. The results showed that 22 PpAux/IAA candidate genes were found in the peach genome. A chromosome mapping analysis revealed that PpAux/IAA genes were distributed with different densities over 5 chromosomes，with the largest number of PpAux/IAA genes on chromosome 1（8 genes）. The DNA-binding and Aux/IAA domains，as well as motif Ⅰ，Ⅱ，Ⅲ and Ⅳ of the PpAux/IAA gene family were highly conserved. The phylogenetic analysis revealed that PpAux/IAA gene family was divided into 10 groups. The results of an intron-exon structure analysis indicated that PpAux/IAA gene family members were composed of 2–5 exons. qRT-PCR results indicated that the expression level of ten PpAux/IAA genes in melting-flesh peach fruits were higher than in stony-hard peach fruits，and the expression level of 5 genes were upregulated in melting-flesh fruits during fruit ripening，especially for ppa010303m，ppa010871m，and ppa020369m. These results suggested that the PpAux/IAA gene family members are highly and structurally conserved，and some of them（especially for ppa010303m，ppa010871m and ppa020369m）are involved in regulating peach fruit ripening.

Key words: peach, Prunus persica, Aux/IAA gene family, fruit ripening, indole-3-acetic-acid

CLC Number:

S 662.1

ZENG Wenfang，WANG Xiaobei，PAN Lei，NIU Liang，LU Zhenhua，CUI Guochao，and WANG Zhiqiang*. Identification and Expression Profiling of Aux/IAA Family Gene During Peach Fruit Ripening[J]. ACTA HORTICULTURAE SINICA, 2017, 44(2): 233-244.

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Identification and Expression Profiling of Aux/IAA Family Gene During Peach Fruit Ripening

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