Effects of Tomato Psyllid Infestation on Activity of Anti-oxidant Enzymes and Expression Levels of Defense Response Related Genes in the Tomato Carrying Resistance Gene Mi-1.2

doi:10.16420/j.issn.0513-353x.2016-0080

ACTA HORTICULTURAE SINICA ›› 2016, Vol. 43 ›› Issue (7): 1286-1294.doi: 10.16420/j.issn.0513-353x.2016-0080

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Effects of Tomato Psyllid Infestation on Activity of Anti-oxidant Enzymes and Expression Levels of Defense Response Related Genes in the Tomato Carrying Resistance Gene Mi-1.2

GANBAATAR Oyunchuluun*，NIU Yi-ding*，BAO Wen-hua，HASI Agula，and HA Da**

（College of Life Science，Inner Mongolia University，Hohhot 010021，China）

Online:2016-07-25 Published:2016-07-25

Abstract

Abstract:

Mi-1.2 is a CC-NBS-LRR protein encoding gene in tomato that confers broad-spectrum resistance toward nematode and piercing-sucking insects such as aphid，whitefly and psyllid. In this study，we investigated the mechanism of Mi-1.2 resistance toward tomato psyllid by measuring the activity of anti-oxidant enzymes and transcript levels of defense response related genes in resistant tomato cultivar‘Motelle’challenged with tomato psyllid infestation. Feeding of tomato psyllid elevated the activity of protective enzymes POD，CAT and APX，and also induced the expression of the gene coding for PAL，a key enzyme in SA synthesis，SA signaling marker gene PR-1 and also three WRKY family genes required for Mi-1.2 mediated defense response. Mi-1.2 itself is also upregulated by tomato psyllid feeding.

Expression level of JA/systemin signaling pathway related genes such as PinII，TPI-1，PR-6，PR-7，PR-5 and prosystemin encoding gene Psy did not change，while the expression of JA synthesis related genes AOS2 and LoxD was repressed by tomato psyllid infestation. The results indicated that SA signaling pathway is involved in Mi-1.2 mediated tomato resistance against piercing-sucking insect tomato psyllid. Expression of those genes mentioned above was not altered by RNAi knockdown of Mi-1.2，suggesting that Mi-1.2 has no regulatory role on the expression of these genes.

Key words: tomato, Mi-1.2, tomato psyllid, anti-oxidant enzyme, defense response, SA signaling pathway

CLC Number:

S 641.2

GANBAATAR Oyunchuluun*，NIU Yi-ding*，BAO Wen-hua，HASI Agula，and HA Da**. Effects of Tomato Psyllid Infestation on Activity of Anti-oxidant Enzymes and Expression Levels of Defense Response Related Genes in the Tomato Carrying Resistance Gene Mi-1.2[J]. ACTA HORTICULTURAE SINICA, 2016, 43(7): 1286-1294.

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Effects of Tomato Psyllid Infestation on Activity of Anti-oxidant Enzymes and Expression Levels of Defense Response Related Genes in the Tomato Carrying Resistance Gene Mi-1.2

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