Abstract: In this study，a population of F2 generation including 180 individual plants was derived from the interspecific cross between the female line Capsicum annuum‘V06C1720’and the male line C. frutescens‘H101’. A genetic linkage map containing 17 linkage groups（LGs）was constructed included 278 markers of SSR and SRAP，and covered 1 282.10 cM with an average marker interval of 4.61 cM. With the phenotypic data of F2 and F2:3 populations，QTLs for 15 main agronomic traits were analyzed by the software of QTLNetwork 2.0 in pepper. A total of 48 additive QTLs and 11 pairs of epistatic QTLs were detected，which located on LG 1，2，3，4，6，7，10，12，13，14，15 and 16，explaining5.18%–40.33% and 4.09%–13.56% of the phenotypic variation，respectively. Thirty-three additive QTLs（68.75%）were major QTLs accounting for more than 10.00% of the phenotypic variation. Twenty-nine additive QTLs alleles（60.42%）from the parent‘H101’toward increasing the trait value were detected. Nine additive QTLs controlling plant height，primary axis length，fruit length，fruit diameter，fruit weight，fruit shape and fruit pungency respectively was persistently expressed in both of F2 and F2:3 populations. The QTL that were consistent in different environments or genetic backgrounds could be useful for marker-assisted selection of agronomic traits，fine mapping and positional cloning.

Key words: pepper, F2 population, F2:3 population, agronomic trait, QTL