Abstract: Black-spot disease is the most serious disease for roses. In order to isolate the genes related with the rose resistance and further to explore the interaction molecule mechanism between rose and Diplocarpon rosae Wolf.‘Pink Peace’as the high resistance variety in the preliminary screening was selected as the material，the cDNA of its inoculated leaves in 24 h，48 h and 72 h were mixed equivalently，and the mixture was regarded as the tester，the cDNA mixture of non-inoculated leaves corresponding hours were used as the driver. Based on these，the subtracted cDNA library were builted. A total of 200 positive clones were picked and? sequenced，and 37 non-redundant sequences were obtained. Among them，32 ESTs showed similarity to known sequences in GenBank. Then，all 37 EST sequences were imported to the database of transcriptome sequencing built with the samples of the same two mixtures，and their RPKM ratios of the inoculated samples to non-inoculated ones were regarded as the gene relative expression. The results showed that the genes including ion transport，reactive oxygen removal，photorespiration and phenylpropanoid pathway and so on were up-regulated in the inoculated samples，and the genes involved in photorespiration，reactive oxygen removal and phenylpropanoid pathway were more，which suggested that multiple genes involved in the rose defending system. Among of them，reactive oxygen removal，photorespiration and phenylpropanoid pathway are playing important roles in rose’s resistance to black spot pathogen.

Key words: rose, black-spot disease, suppression subtractive hybridization, reactive oxygen removal, photorespiratory, phenylpropanoid pathway