园艺学报 ›› 2019, Vol. 46 ›› Issue (4): 775-783.doi: 10.16420/j.issn.0513-353x.2018-0561

• 研究报告 • 上一篇    下一篇



  1. 中国热带农业科学院南亚热带作物研究所,海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江 524091
  • 出版日期:2019-04-25 发布日期:2019-04-25
  • 基金资助:

Lignification Induced by Ethephon and Related Gene Expression in Postharvest Flowering Chinese Cabbage at Low Temperature

SONG Kanghua,JIA Zhiwei,CHANG Jinmei,SUN Manli,and ZHANG Lubin*   

  1. South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products,Zhanjiang,Guangdong 524091,China
  • Online:2019-04-25 Published:2019-04-25

摘要: 为了探讨低温条件下乙烯对采后菜薹木质化的影响,以‘四九菜薹’为试材,分别用不同浓度(100、200 和500 μL · L-1)乙烯利处理,置于1 ℃低温下贮藏,并于0、24、48、72 和96 h取样,进行木质素含量、相关酶活性测定以及相关基因表达分析。结果表明:不同浓度乙烯利处理均提高了菜薹茎部的木质素含量和木质素合成途径中4–香豆酸辅酶A连接酶(4-CL)、过氧化氢酶(POD)的活性;200 μL · L-1乙烯处理提高了2个乙烯响应因子(ERF)BcERF2、BcERF1B以及2个POD基因BcPOD67、BcPOD69的表达。乙烯利处理促进了采后菜薹木质化的进程,推测乙烯可能通过乙烯响应因子BcERF2和BcERF1B调控BcPOD69、BcPOD67等参与木质素的生物合成。

关键词: 菜薹, 乙烯, 木质化, ERF, POD, 马铃薯, 植原体病害, 分类, 功能基因, 抗病育种

Abstract: The purpose of this study was to investigate the effects of ethephon on the stem lignification of postharvest flowering Chinese cabbage(FCC,Bcassica campestris L. ssp. chinensis var. utilis Tsen et Lee)stored at low temperature. The postharvest FCC were treated with different concentrations of ethephon(100,200 and 500 μL · L-1),placed in an incubator at 1 ℃. The control was treated with distilled water. The content of lignin and activity of enzymes(4-coumaric acid coenzyme A ligase,4-CL;peroxidase,POD;cinnamate dehydrogenase,CAD)related to lignification were detected by spectrophotometer. The gene expression levels of BcERF2,BcERF1B,BcPOD67 and BcPOD69 were evaluated by real-time PCR. The results showed that the content of lignin in the treated FCC stems significantly increased and the activities of POD and 4-CL also showed a rising trend during storage. With the increase of ethephon concentration,the content of lignin and activities of POD and 4-CL gradually increased. The expression levels of BcERF2,BcERF1B,BcPOD67 and BcPOD69 were higher than the control,which were consistent with the lignin change in treated FCC with ethylene(200 μL · L-1)during storage. In summary,the ethephon treatment accelerated the lignification process of postharvest FCC at low temperature. The ethephon was involved in lignin biosynthesis,which may through the regulation BcPOD67 and BcPOD69 by transcription factors BcERF2 and BcERF1B.

Key words: flowering Chinese cabbage, ethylene, lignification, ERF, POD, potato, phytoplasma disease, taxonomy, function genes, disease resistant breeding