园艺学报 ›› 2018, Vol. 45 ›› Issue (6): 1185-1192.doi: 10.16420/j.issn.0513-353x.2017-0672

• 研究报告 • 上一篇    下一篇


王贵芳1,于 雯2,罗静静2,肖元松2,赵永飞2,陈晓璐2,彭福田2,*   

  1. 1山东省果树研究所,山东泰安 271000;2山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018
  • 出版日期:2018-06-25 发布日期:2018-06-25
  • 基金资助:

Effects of Overexpression of Pingyitiancha MhSnRK1 on Tomato Seed Germination and Seedling Growth

WANG Guifang1,YU Wen2,LUO Jingjing2,XIAO Yuansong2,ZHAO Yongfei2,CHEN Xiaolu2,and PENG Futian2,*   

  1. 1Shandong Institute of Pomology,Tai’an,Shandong 271000,China;2College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,Tai’an,Shandong 271018,China
  • Online:2018-06-25 Published:2018-06-25

摘要: 为探讨蔗糖非发酵–1–相关蛋白激酶–1(SnRK1)对种子萌发及幼苗生长的调控作用,以超表达平邑甜茶MhSnRK1的番茄株系O-7、O-9和其野生型(WT)为试材,研究MhSnRK1对番茄种子萌发及幼苗生长发育的影响。结果表明,WT、O-7和O-9在MS培养基中种子发芽率均为100%,在MS + 100 mmol · L-1海藻糖培养基中发芽率显著降低,分别为23.3%、70.3%和67.7%;在MS培养基中O-7和O-9的SnRK1活性分别比WT高12.1%和2.3%,在海藻糖培养基中WT、O-7和O-9比在MS培养基中分别降低41.5%、35.5%和27.5%,O-7和O-9仍高于WT,可见在一定范围内较高的SnRK1活性促进种子的萌发。播种后14 d,与其他培养基中相比,海藻糖培养基中幼苗主根的长度最短,其中WT显著短于O-7和O-9;幼苗下胚轴的伸长较MS培养基中明显受到抑制,且WT受抑制的程度更大;在海藻糖培养基中添加0.3 mg · g-1的IAA,WT下胚轴伸长受到的抑制得到解除。播种后30 d,海藻糖培养基中,与O-7和O-9相比,WT表现为子叶平整肥厚,真叶发育良好,叶面积较大,叶绿素含量显著高于 O-7和O-9。上述结果表明,平邑甜茶MhSnRK1在番茄中超表达调控种子的萌发及幼苗的生长;在一定范围内,较高的SnRK1活性促进种子的萌发、抑制幼苗主根和下胚轴的伸长,较低的SnRK1活性更有利于幼苗的生长发育。

关键词: 平邑甜茶, MhSnRK1, 番茄, SnRK1活性, 种子发芽率, 下胚轴

Abstract: This study aims to examine the effects of sucrose non-fermenting-1-related protein kinase 1(SnRK1)from Malus hupenhensis Rehd.(Pingyitiancha)on seed germination and seedling growth in tomatoes. The germination and growth of two tomato lines overexpressing Malus hupenhensis SnRK1 (MhSnRK1),O-7 and O-9,as well as their wild-type counterpart(WT),were monitored in six different mediums. The results showed that,the germination rates of the three tomato lines were all 100% in MS medium but declined to varying degrees in trehalose medium,with WT showing the lowest germination efficiency(WT,23.3%;O-7,70.3%;O-9,67.7%). Meanwhile,the SnRK1 activities of O-7 and O-9 cultivated in MS were 12.1% and 2.3% higher,respectively,compared to that of WT. The addition of trehalose led to a 41.5% reduction in the activity of SnRK1 in WT,35.5% in O-7 and 27.5% in O-9. Fourteen days after sowing,trehalose was found to significantly suppress the growth of taproot and hypocotyl in all three tomato lines,with the inhibitory effect more pronounced on WT than on the two transgenic tomato lines. The introduction of 0.3 mg · g-1 IAA was shown to mitigate the inhibitory effect of trehalose on hypocotyl elongation in WT. As a result,in MS medium,WT displayed flatter,flesher and larger cotyledon,as well as better-developed true leaf,compared to O-7 and O-9 at day 30 after sowing. And the leaf chlorophyll content of WT was significantly higher than that of O-7 and O-9. These results showed that overexpressing MhSnRK1 in tomato could regulate the seed germination and seedling growth. In a certain range,the higher SnRK1 activity promoted the seed germination,inhibited the taproots and hypocotyls of seedling elongating,while the lower SnRK1 activity is more conducive to the growth and development of the seedlings.

Key words: Malus hupenhensis, MhSnRK1, tomato, SnRK1 activity, seed germination rate, hypocotyl