园艺学报 ›› 2016, Vol. 43 ›› Issue (6): 1079-1088.doi: 10.16420/j.issn.0513-353x.2016-0240

• 蔬菜 • 上一篇    下一篇


吴俊清,赵 静,秦美玲,任延靖,张华敏,代子卉,郝玲玉,张鲁刚*   

  1. (西北农林科技大学园艺学院,农业部西北地区园艺作物生物学与种质资源创新重点实验室,陕西杨凌 712100)
  • 出版日期:2016-06-25 发布日期:2016-06-25

Genetic Analysis and Primary Mapping of the Purple Gene in Purple Heading Chinese Cabbage

WU Jun-qing,ZHAO Jing,QIN Mei-ling,REN Yan-jing,ZHANG Hua-min,DAI Zi-hui,HAO Ling-yu,and ZHANG Lu-gang*   

  1. (College of Horticulture,Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China of Ministry of Agriculture,Northwest A & F University,Yangling,Shaanxi 712100,China)
  • Online:2016-06-25 Published:2016-06-25


大白菜[Brassica rapaLour.ssp. pekinensis]紫心纯系‘14S839和橙色纯系‘14S162为亲本,构建F2群体,对大白菜紫色基因(BrPur)进行遗传连锁分析。性状分离调查结果显示,在F2群体中紫心与非紫心单株符合31分离比例,说明大白菜紫心性状的有、无符合单基因显性遗传模式,紫心性状是一种显性性状。采用改良的BSA方法对紫色基因BrPur进行定位,经过对白菜全基因组297SSR标记的筛选,得到了BrPur的两个侧翼标记A710A714,同时结合白菜基因组信息,将BrPur定位于大白菜A07连锁群上。结合前人关于花青素代谢相关基因的研究报道,通过分析Br4CL3 Bra004214的序列、开发新的标记,得到了两个特异共显性标记CL-12B214-87。利用F2群体扩增验证筛选到的所有标记,结果表明,Join Map 4.0作图得到的遗传连锁图与非紫色交换单株“基因型矩阵”显示的结果完全一致,标记CL-12B214-87位于BrPur两侧,遗传距离分别是3.1 cM3.5 cM

关键词: 大白菜, 紫心叶球, 分子标记, 连锁分析, 基因定位


To analyze genetic linkage and map the location of purple geneBrPurcontrolling purple inner leaves trait of Chinese cabbagea F2 population constructed by the single-plant-selfing of F1 hybridized from homozygous purple heading line14S839and homozygous orange heading line14S162was used. The field investigation of head color shows that the separation proportion of purple heading individuals to non-purple heading individuals fitted to the ratio of 31 in F2 populationwhich means that the purple heading of Chinese cabbage was a dominant phenotype and consistent with genetic pattern of a single dominant gene. Thentwo flanking linkage markersA710 and A714were obtained by a modified BSA method with 297 SSRs in whole genomeand the BrPur was primarily mapped on linkage group A07 based on the location of the two flanking markers sequence in BRAD. Subsequentlytwo novel flanking markersCL-12 and B214-87were developed by cloning and homology sequence alignment analysis of Br4CL3 and Bra004214 involved in anthocyanin related genes in previous reports. Finallywe verified the linkage relationship of all markers using F2 populationand found that their genetic map obtained by Join Map 4.0 was consistent with the result from Plant Genotype Matrix of all non-purple crossover individualswhich placed CL-12 and B214-87 flanking two side of BrPur locus at genetic distances of 3.1 cM and 3.5 cMrespectively. 

Key words: Chinese cabbage, purple heading leaf, molecular marker, linkage analysis, gene mapping