园艺学报 ›› 2015, Vol. 42 ›› Issue (11): 2183-2196.doi: 10.16420/j.issn.0513-353x.2015-0436

• 蔬菜 • 上一篇    下一篇

辣椒全基因组WRKY 转录因子的分析

刁卫平,王述彬*,刘金兵,潘宝贵,郭广君,戈 伟   

  1. 江苏省农业科学院蔬菜研究所,江苏省高效园艺作物遗传改良重点实验室,南京 210014
  • 出版日期:2015-11-25 发布日期:2015-11-25
  • 基金资助:

    国家高技术研究发展计划(‘863’计划)项目(2012AA100103);江苏省自然科学基金项目(2014 BK1380);国家现代农

Genome-wide Analysis of the WRKY Transcription Factor Family in#br# Pepper

DIAO Wei-ping,WANG Shu-bin*,LIU Jin-bing,PAN Bao-gui,GUO Guang-jun,and GE Wei   

  1. Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Jiangsu Key Laboratory for Horticultural Crop
    Genetic Improvement,Nanjing 210014,China
  • Online:2015-11-25 Published:2015-11-25


基于已公布的辣椒全基因组数据,利用生物信息学方法对辣椒WRKY 转录因子家族进行全
研究。结果表明:辣椒CaWRKY 家族包含71 个基因,根据WRKY 结构域的数量及锌指结构的特征可将
其分为GroupⅠ、GroupⅡ和GroupⅢ等3 大类,GroupⅡ又可分为Ⅱ(a)、Ⅱ(b)、Ⅱ(c)、Ⅱ(d)和
Ⅱ(e)等5 个亚类。辣椒12 条染色体上均有WRKY 转录因子分布,其中第1 号染色体上分布最多,共
有10 个,第4 号染色体上分布最少,仅有2 个。辣椒每类/亚类WRKY 几乎含有相同的保守基序。辣椒
WRKY 编码的蛋白在132 ~ 869 个氨基酸范围内,平均氨基酸数量为373 个。

关键词: 辣椒, WRKY, 转录因子, 生物信息学


In the present study,based on the recently released pepper whole-genome sequences,
CaWRKY gene family identification,gene classification,chromosome location,sequence alignment and
conserved structure domains of CaWRKY proteins were predicted and analyzed with bioinformatics
methods. The results showed that 71 CaWRKY genes were identified which were classified into three main
groups(Ⅰ,Ⅱand Ⅲ),with the second group further divided five subgroups[Ⅱ(a),Ⅱ(b),Ⅱ(c),
Ⅱ(d)and Ⅱ(e)]. A total of 70 CaWRKY genes were mapped to 12 chromosomes,whereas only
CaWRKY70 was not mapped to any particular chromosome. Genome mapping analysis revealed that
pepper WRKY genes were enriched on several chromosomes(1,2,3 and 7),especially on chromosome 1
which encompasses the largest number of 10 CaWRKY genes,while chromosome 4 only contained 2
CaWRKY genes. The pepper WRKYs from each group or subgroup were shown to share similar motif
compositions,and CaWRKY proteins contained 132–869 amino acids and 373 in average. Our results will
provide a platform for functional identification and molecular breeding tudy of WRKY genes in pepper.

Key words: pepper, WRKY, transcription factor, bioinformatic