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园艺学报 ›› 2015, Vol. 42 ›› Issue (6): 1093-1102.doi: 10.16420/j.issn.0513-353x.2015-0048

• 蔬菜 • 上一篇    下一篇

几份甘蓝蜡粉缺失突变体特征特性的研究

唐俊,刘东明,刘泽洲,杨丽梅,方智远,刘玉梅,庄木,张扬勇,孙培田   

  1. 中国农业科学院蔬菜花卉研究所,北京 100081
  • 出版日期:2015-06-25 发布日期:2015-06-25
  • 基金资助:
    国家‘863’计划课题(2012AA100101);农业部园艺作物遗传改良重点开放试验室项目;国家现代农业产业技术体系建设专项资金项目(CARS-25-B-01);‘十二五’国家科技支撑项目(2012BAD02B01)

Studies on Characteristics of Several Glossy Mutants in Cabbage

TANG Jun, LIU Dong-Ming, LIU Ze-Zhou, YANG Li-Mei, FANG Zhi-Yuan, LIU Yu-Mei, ZHUANG Mu, ZHANG Yang-Yong, SUN Pei-Tian   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2015-06-25 Published:2015-06-25

摘要: 为了探索甘蓝蜡粉缺失突变体的特征特性和实际应用价值,对不同类型的5份甘蓝蜡粉缺失突变体进行了研究。叶表超微结构观察及蜡粉成分分析发现:突变体叶表面蜡粉严重缺失,其蜡粉量仅约为野生型的30%;野生型叶表蜡粉晶体结构主要为柱状和线状,而突变体主要为颗粒状和短棒状;野生型叶表蜡粉成分主要为烷烃、脂肪酸、醇、醛和酮,而突变体主要缺失成分为烷烃和酮,推测突变体蜡粉合成途径中的烷烃合成途径受阻,从而表现为蜡粉缺失。突变体的叶绿素浸出率和叶片失水率均显著高于其野生型,说明其叶片角质层透性增加。植株生长曲线的测定结果显示:突变体1和突变体3与其野生型相比生长相对滞后,生长势稍弱,而突变体2的生长与其野生型无显著差异。农艺性状及杂种优势的调查结果表明:蜡粉缺失甘蓝叶色亮绿,商品性好,特别是突变体2,不仅球形美观且其蜡粉缺失性状为显性遗传;用纯合显性的突变体2的自交系与普通材料配制的杂交组合具有球色亮绿、杂种优势明显的优点,实际应用价值较大。

关键词: 结球甘蓝, 蜡粉缺失突变体, 超微结构, 蜡粉成分, 农艺性状, 杂种优势

Abstract: To detect the characteristics and practical application potential of glossy mutants in cabbage,five different glossy mutants were studied. The epicuticular wax amount over these mutants was just about 30% of their wild type. The wax crystal structure of wild type was columnar and linear,but the wax crystal structure of mutant was granular and short rod. The wax composition of wild type cabbage was mainly consisted of alkanes,alcohols,fatty acids,ketones,and aldehydes,while in the mutant there were severely lack of alkanes and ketones. We speculated that the biosynthetic pathway of alkane in the mutant was blocked,so it exhibited the glossy traits. In addition,cuticle permeability was obvious increased in glossy mutant. The results of growth curve indicated that the growth speed of mutant-1 and mutant-3 was slower than their wild type,and the plant size of mutant-1 and mutant-3 was smaller than the correspondingwild type too,but the glossy traits has no influence on the growth of mutant-2. The study on agronomic traits and heterosis of the glossy mutants showed that the color was bright green and the glossy mutants exhibited better commodity nature and the inheritance of mutant-2 was dominant. Besides,the bright green F1 cabbage has the advantage of heterosis indicating that mutant-2 possessed potential value for practical application.

Key words: cabbage, glossy mutant, microstructure, wax component, agronomic trait, heterosis

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