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园艺学报 ›› 2008, Vol. 35 ›› Issue (9): 1305-1309.

• 蔬菜 • 上一篇    下一篇

茄子单性结实基因的遗传分析及AFLP分子标记

刘富中1*;万 翔1,2;陈钰辉1;连 勇1; 宋 明3   

  1. 1中国农业科学院蔬菜花卉研究所,北京 100081;2四川资阳农业局,四川资阳641300;3西南大学园艺园林学院,重庆400716)
  • 收稿日期:2008-06-23 修回日期:2008-08-08 出版日期:2008-09-25 发布日期:2008-09-25
  • 通讯作者: 刘富中

Inheritance of the Eggplant Parthenocarpy and AFLP Molecular Marker

LIU Fu-zhong1*, WAN Xiang1,2,CHEN Yu-hui1, LIAN Yong1, and SONG Ming3   

  1. (1Institute of Vegetables & Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081,China;2the Ziyang County office of Agricultural, Ziyang ,Sichuan 641300, China;3Department of HorticuLture, Southwest University, Chongqing 400716,China)
  • Received:2008-06-23 Revised:2008-08-08 Online:2008-09-25 Published:2008-09-25
  • Contact: LIU Fu-zhong

摘要: 以茄子单性结实自交系D-10和非单性结实自交系03-2为试验材料,研究了单性结实基因的遗传特性及其AFLP标记。对杂交后代F1、F2、BC1单性结实性表现型分离比例的分析表明,茄子D-10的单性结实性由单显性核基因控制,其基因用符号Pat表示。采用AFLP分析技术和改良BAS法,通过512对E/M引物组合的筛选,获得1个与茄子单性结实基因紧密连锁的AFLP标记E75/M53-70,该标记与单性结实基因间的遗传图距为15.38 cM,可用于茄子单性结实性的鉴定和单性结实分子标记辅助育种,加速茄子单性结实基因的转育和利用。

关键词: 茄子, 单性结实基因, 遗传规律, AFLP标记

Abstract: Emphytic character and AFLP marker of eggplant parthenocarpic gene were studied by using materials of parthenocarpic selfing line D-10 and un-parthenocarpic selfing line 03-2. Analysing the separated rate of the parthenocarpic phenotype in F1,F2, and BC1, we found that parthenocarpic gene of D-10 was controlled by single dominant nucleus gene and named as Pat. Using AFLP and BSA techniques with 512 pairs of E/M primers screening, we obtained one AFLP marker E75/M53-70 that linked with parthenocarpic gene.The genetic distance was 15.38 cM,which could be used as parthenocarpic identification and molecular marker-assisted selection.

Key words: eggplant, parthenocarpic gene, genetic regulation, AFLP molecular marker

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