牡丹栽培品种的RAPD分析

园艺学报 ›› 2001, Vol. 28 ›› Issue (4): 370-372.

牡丹栽培品种的RAPD分析

陈向明¹;郑国生^{2 ,*};张圣旺²

(¹ 合肥教育学院, 合肥230001 ;² 山东农业大学生命科学学院, 泰安271018)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-08-25 发布日期:2001-08-25

RAPD Analysis of Tree Peony Cultivars

Chen Xiangming¹ , Zheng Guosheng² , and Zhang Shengwang²

(¹ Hefei Education College , Hefei 230001;　²Life Science College , Shandong Agricultural University , Tai’an 271018)

Received:1900-01-01 Revised:1900-01-01 Online:2001-08-25 Published:2001-08-25

摘要/Abstract

摘要： 对7 种花色系的35 个牡丹栽培品种进行RAPD 分析, 34 个随机引物共扩增出418 个位点, 其中337 个(80. 62 %) 多态位点表明受试品种间丰富的遗传多态性。同一花色的不同品种间和不同花色系间都存在较大的遗传多态性, 不同花色系间多态性位点频率大小依次排序为蓝色系> 黄色系> 深红色系> 黑色系> 白色系> 绿色系> 红色系; 利用UPGMA 软件进行聚类分析, 35 个品种被分为5 个遗传聚类组, 除红色系外, 其它6 种花色与遗传聚类组的划分无必然相关性。

关键词: 牡丹, 品种, 基因组, 遗传多样性, RAPD

Abstract: The genomic diversity of thirty-five tree peony cultivars of 7 different flower color were analised by RAPD. The results showed that 337 (80. 62 %) polymorphic sites were detected among the total 418 sites amplified indicating abundant genomic diversity of tree peony cultivars. Similar results were also revealed between accessions within the same flower color series , but significant differences were observed between different flower color series in the order as Blue series > Yellow > Dark red > Black >White > Green > Red ones based upon the percentage of the polymorphic sites.
The genetic distance and genetic relationships of the accessions were constructed through unweighted paired group method with arithmetic averages cluster analysis , all accessions were grouped into 5 genetic types , no significant correlation between flower color series and genetic types were detected except Red flower series.

Key words: Tree peony cultivar, Genome, Genetic diversity, RAPD

中图分类号:

S 68

陈向明;郑国生;张圣旺. 牡丹栽培品种的RAPD分析[J]. 园艺学报, 2001, 28(4): 370-372.

Chen Xiangming;Zheng Guosheng;and Zhang Shengwang. RAPD Analysis of Tree Peony Cultivars[J]. ACTA HORTICULTURAE SINICA, 2001, 28(4): 370-372.

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