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园艺学报 ›› 2002, Vol. 29 ›› Issue (1): 72-74.

• 研究报告 • 上一篇    下一篇

花椰菜类蔬菜自交系基因组间亲缘关系的AFLP分析

孙德岭1;赵前程2;宋文芹1;陈瑞阳1
  

  1. (1南开大学生命科学学院, 天津300192;2天津市蔬菜研究所, 天津300384)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-02-25 发布日期:2002-02-25

Analysis of Relationships of Cauliflower, Broccoli and Purple Cauliflower byAFLP

Sun Deling1, Zhao Qiancheng2, Song Wenqin1, and Chen Ruiyang1
  

  1. (1College of Life sciences , Nankai University , Tianjin 300192; 2Tianj in Vegetable Research I nstitute , Tianj in 300384)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-02-25 Published:2002-02-25

摘要: 利用AFLP 技术对花椰菜、青花菜、紫花菜、黄花菜自交系的遗传亲缘关系进行了研究。使
用8 对选择性引物组合, 扩增出4901 条电泳带, 并对其进行聚类分析, 根据UPGMA 方法构建聚类树状图。结果表明: 花椰菜、青花菜、紫花菜、黄花菜明显分为四大类群; 青花菜与紫花菜亲缘关系较近, 花椰菜与其它花菜亲缘关系较远, 黄花菜居中。

关键词: ALFP, 亲缘关系, 花椰菜, 青花菜

Abstract: Genetic relationships of inbreed line of cauliflower , broccoli and purple cauliflower were examimed by AFLP technology. 4901 bands were detected by using eight pairs of selective primers. Clustering analysis showed: cauliflower, broccoli, purple cauliflower and yellow cauliflower were divided into four groups, as to hered itary distance, it is close between purple cauliflower and broccoli, cauliflower is distant from others, yellow cauliflower is in midst. The art icle analysed relationships of cauliflower on molecular lever, and the method of analysing relat ionships of cauliflower with AFLP was studied.

Key words: AFLP, Relationships, Cauliflower, Broccoli

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