园艺学报 ›› 2003, Vol. 30 ›› Issue (6): 728-730.

• 研究报告 • 上一篇    下一篇


田景花;张 红;李 明;王 梅   

  1. (河北农业大学园艺学院,保定071001)
  • 收稿日期:2002-10-09 修回日期:2002-12-06 出版日期:2003-12-25 发布日期:2003-12-25

Immunity Coloidal Gold Localization of Calmodulin in Cucumber Young LeafCells

Tian Jinghua,Zhang Hang,Li Ming, and Wang Mei   

  1. (College ofHorticulture,Hebei Agricultural University,Baoding 071001, China)
  • Received:2002-10-09 Revised:2002-12-06 Online:2003-12-25 Published:2003-12-25

摘要: 采用免疫胶体金电镜技术对黄瓜幼叶叶肉细胞中的钙调素(CaM)进行定位。结果表明:适

关键词: 黄瓜, 钙调素, 免疫胶体金, 透射电镜技术

Abstract: Using glutaraldehyde an d parafonnaldehyde as fixative an d Lowicryl K4M as embedding medium,the immunity colloidal gold localization of calmodulin (CaM ) in cucunber young leaf cells Was investigated by transmission electron mi croscope. The results were as follows: at the optimal growth temperature, CaM mainly distributed in nucleus an d chloroplast. In addition, there Was some CaM in mitoehondria, a little in cytoplasm, vac
uole,tonoplast an d plasma membrane. However, it Was difficult to find CaM in cell wall an d intercelular space.

Key words: Cucumber, Calnlodulin, Immunity colloidal gold, Transmi ssion electron microscope