园艺学报 ›› 2003, Vol. 30 ›› Issue (6): 725-727.

• 研究报告 • 上一篇    下一篇


陈劲枫 罗向东 钱春桃 曹清河   

  1. (作物遗传与种质创新国家重点实验室,南京农业大学蔬菜研究所,南京210095)
  • 收稿日期:2002-10-24 修回日期:2003-01-27 出版日期:2003-12-25 发布日期:2003-12-25

Production and Characterization of M onosomic Alien Addition Lines in Cucum ber

Chen Jinfeng,Luo Xiangdong,Qian Chuntao,and Cao Qinghe   

  1. (State LaboratoD,of Crop Genetics and Germplasm Enhancement, Vegetable Research Institute,Nanjing Agricultural Uruversity,Nanjing 210095, China)
  • Received:2002-10-24 Revised:2003-01-27 Online:2003-12-25 Published:2003-12-25

摘要: 将甜瓜属异源三倍体与栽培黄瓜‘北京截头’杂交,经胚胎拯救获得了两个2n=15 (14C+
1H)的单体异附加系,编号分别为02—17和02—39。02一l7植株叶形为戟形,02—39为深陷的掌状形,并且果刺均为白色,不同于异源三倍体的掌状心脏形和黑刺,果形比异源三倍体黄瓜长而更接近普通黄瓜。细胞学研究发现,单体异附加系在终变期和中期I染色体构型主要是由7个二价体和1个单价体构成,出现三价体的频率为4% ~5% ,中期Ⅱ不均等分裂形成不同的两极,一极染色体为8条,另一极为7条。RAPD分析证明它们分刖附加了野生种Cucum/s hystrix的染色体。

关键词: 黄瓜, Cucumis hystrix, 单体异附加系

Abstract: Two monosomic alien addition lines 02.17 and 02.39 (2n:15. 14C+1H)were identified among 52 plan ts derived from embryo rescue of an Cucumis allotriploid× cucumber. Diferent from their allotriploid parent, the plan ts have white spine, an d the shape of leaves in 02.17 an d 02.39 was has tate an d palmate respectively.In pollen mother cells (PMCs) of 02.1 7 and 02.39.7 bivalents and 1 univalent were observed at diakinesis and metaphase i.Low frequency (about 4% 一5% )of trivalents has also been observed at metaphase JI.At metaphase 11,8 chromosomes went to one pole while 7 to another.R APD analysis indicated these two plants were added with one alien chromosome from C.hystrix.

Key words: Monosomie alien addition lines, Cucumber, Cucumis hystrix