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园艺学报 ›› 2010, Vol. 37 ›› Issue (1): 31-38.

• 果树 • 上一篇    下一篇

澳洲坚果不同品种耐寒特性的研究

岳 海1 ;李国华1*; 李国伟2 ;陈丽兰1 ;孔广红1;梁国平1   

  1. (1 云南省热带作物科学研究所, 云南景洪666100; 2 云南天然橡胶产业股份有限公司勐捧分公司, 云南勐腊663300)
  • 收稿日期:2009-06-10 修回日期:2009-11-16 出版日期:2010-01-25 发布日期:2010-01-25
  • 通讯作者: 李国华

Studies on Cold Resistance of Different Macadamia Cultivars

YUE Hai1 ;L I Guo-hua1*; LI Guo-wei2 ;CHEN L i-lan1 ;KONG Guang-hong1 ; LIANG Guo-ping1   

  1. (1 Yunnan Institute of Tropical Crops, Jinghong, Yunnan 666100, China; 2Yunnan Natural Rubber Industrial Co. , L td. Mengpen B ranch, Mengla, Yunnan 663300, China)
  • Received:2009-06-10 Revised:2009-11-16 Online:2010-01-25 Published:2010-01-25
  • Contact: LI Guo-hua

摘要: 以3个澳洲坚果(Macadamia integrifolia Marden & Betche) 品种(H2、HAES900和OC) 幼苗为试材, 研究了自然低温胁迫对其叶片形态, 相对电导率(REC) , 叶绿素、丙二醛(MDA) 、游离脯氨酸( Pro) 和可溶性蛋白含量, 超氧化物歧化酶( SOD) 活性的影响。结果表明: 自然低温处理下, HAES900品种叶片产生较多水渍状斑, 叶脉处水渍状斑连在一起, 最终导致叶片及植株死亡; OC品种叶片水渍状斑较少, H2品种只在叶片表皮出现一些褐斑, 基本没有寒害症状。自然低温下, H2和OC品种叶片MDA积累增多, 相对电导率升高, 叶绿素含量下降, 其中叶绿素a下降程度较大, 但随着脯氨酸和可溶性蛋白含量的升高及SOD酶活性的增强, MDA积累升高变缓且有下降趋势; 胁迫解除后, 各指标趋向于处理前水平。而HAES900品种在低温处理3 d后, 相对电导率显著升高, 蛋白质和脯氨酸含量开始下降, SOD酶活性降低, 随着胁迫的进一步加重(6~9 d) , MDA积累量增大, 产生不可逆的生理变化, 即使解除胁迫后各指标变化趋势不变。

关键词: 澳洲坚果, 叶绿素, 超氧化歧化酶, 可溶性蛋白, 抗寒性

Abstract: The seedlings of three macadamia cultivars were used as experimental material to study their cold resistance. The changes in leaf morphology, relative electric conductivity ( REC) , malondialdehyde(MDA) content, leaf chlorophyll content, free proline content, soluble protein content, and superoxide dismutase activity ( SOD) were measured to evaluate their resistance to low temperature stress. Three macadamia cultivar seedlings showed different degrees of leaf damage symptom. HAES900 produced more frozen spots than H2 and OC. The frozen spots close to leaf vein connected, leading to death of leaves and even the whole plant. H2 only showed slightest injury symptom with some browning spots on leaf epidermis. In OC and H2,under low temperature stress, MDA contents and REC increased significantly, while chlorophyll content decreased, with chlorophyll a decreased more significantly than chlorophyll b. However, with the increasing of content of MDA, free proline content and SOD activity increased, and MDA accumulation became very slow or downtrend. After low temperature stress was relieved, the physiological indexes tended to resume to the pre-cold level. For HAES900, after being exposed to low temperature treatment for three days, REC increased significantly, content of soluble protein began to decrease, and the activity of SOD decreased. With the elongation of cold stress (6 - 9 d) , irreversible physiological changes occurred. Even after the relieve of low temperature stress, no recovery change appeared in physiological index.

Key words: macadamia, chlorophyll, superoxide dismutase, soluble protein, cold resistance

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