关键词: 山葡萄, 低温伤害, 叶绿素荧光, 活性氧

Abstract:

The parameters of chlorophyll fluorescence and reactive oxygen metabolism were measured in two different cold-tolerant amur grape cultivars of‘Shuangfeng’（intraspecific hybridization）and‘Zuoyouhong’（interspecific hybridization）. The chlorophyll content，Chl.a/b and Fv/Fm values of the two cultivars were significantly decreased，while the value of 1–qp and MDA content increased significantly with the temperature decreasing gradually. Stable NPQ value and the higher SOD and APX activities in‘Shuangfeng’leaf showed the lower damage degree than‘Zuoyouhong’. The results suggested that the leaf chlorophyll decomposition，electron transfer blocked of PSⅡand excitation energy increased under the low temperature stress eventually resulted in the lipid peroxidation of chloroplast membrane. Thermal dissipation and antioxidant enzymes were helpful to reduce the damage of reactive oxygen to PSⅡ. Therefore，the differences in NPQ and the activities of SOD and APX might be the important aspects that caused the genotype differences in cold injury.

Key words: Vitis amurensis, cold injury, chlorophyll fluorescence, reactive oxygen species