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园艺学报 ›› 2008, Vol. 35 ›› Issue (8): 1199-1204.

• 研究简报 • 上一篇    下一篇

海南椰子栽培品种的SSR标记分析

柳晓磊1;汤 华1;李东栋2*;王 茜1;林艳青1;周 蓉1   

  1. 1海南大学农学院生物技术系, 海口 570228; 2 海南大学理工学院生物工程系, 海口570228)
  • 收稿日期:2008-04-24 修回日期:2008-07-03 出版日期:2008-08-25 发布日期:2008-08-25
  • 通讯作者: 李东栋

Genetic Diversity of Coconut Cultivars from Hainan Province based on SSR Molecular Markers

LIU Xiao-lei1, TANG Hua1, LI Dong-dong2*, WANG Qian1, LIN Yan-qing1, and ZHOU Rong1   

  1. (1Department of Biotechnology, College of Agriculture, Hainan University, Haikou, 570228, China; 2Department of Bioengineering, Hainan University, Haikou, 570228, China)
  • Received:2008-04-24 Revised:2008-07-03 Online:2008-08-25 Published:2008-08-25
  • Contact: LI Dong-dong

摘要:

应用简单重复序列(SSR)标记方法,对海南的11个椰子栽培品种进行了遗传多样性分析。选取30对引物用于PCR扩增,有23对引物扩增出有效多态性片段136条,平均多态性百分率为95.77%,每对引物扩增出的带数2~12条不等,平均为6.17条,每个SSR位点的多态信息量(PIC)变化于0.173~0.896之间,平均为0.561。11份材料之间遗传相似系数变化范围0.061~0.861,说明海南椰子栽培品种之间存在丰富的遗传多样性。UPGMA聚类分析结果表明,11个椰子栽培品种分为四个类群和两个亚群。SSR标记反映出的品种间亲缘关系与形态学研究的分类结果并不完全吻合。

关键词: 椰子, SSR, 遗传多样性

Abstract: In this study, genetic diversity and relationship among 11 coconut (Cocos nucifera L.) cultivars were analyzed based on the data of SSR. Genetic diversity of 11 coconut cultivars from Hainan province were studied using 30 SSR primer pairs, among them, 23 primer pairs amplified polymorphic fragments. A total of 136 polymorphic segments were produced, with the average polymorphic bands per SSR primer pair being 6.17 and ranging from 2 to 12. Polymorphic index content (PIC) values varied from 0.173 to 0.896 with an average of 0.561. The genetic similarity coefficients among 11 coconut cultivars ranged from 0.061 to 0.861. It indicated that there was sufficient genetic diversity among these coconut cultivars. Cluster analysis was made with unweighted pair group method for arithmetic averages (UPGMA) using genetic similarity coefficients. Eleven materials were clustered into 4 groups and 2 sub-groups. The denerogram revealed that traditional morphological classification system could not completely reflect the genetic relationship among coconut cultivars native to Hainan.

Key words: coconut, Cocos nucifera L., SSR, genetic diversity

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