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园艺学报 ›› 2008, Vol. 35 ›› Issue (3): 443-446.

• 研究简报 • 上一篇    下一篇

彩色马蹄莲多倍体的诱导

吴红芝; 张锡庆;郑思乡*; 石景峰;毕玉芬   

  1. (云南农业大学园林园艺学院 昆明 650201)
  • 收稿日期:2007-11-06 修回日期:2008-03-04 出版日期:2008-03-25 发布日期:2008-03-25
  • 通讯作者: 郑思乡

Polyploid Induction of coloured Zantedeschia aethiopica

Wu Hong-zhi, Zhang Xi-qing, Zheng Si-xiang*, Shi Jing-feng and Bi Yu-fen   

  1. (College of Landscape and Horticultural, Yunnan Agricultural University, Kunming 650201, china)
  • Received:2007-11-06 Revised:2008-03-04 Online:2008-03-25 Published:2008-03-25
  • Contact: Zheng Si-xiang

摘要:

在离体培养条件下,比较了不同浓度、不同处理时间的秋水仙素诱导彩色马蹄莲体细胞染色体加倍的效果。经形态学和气孔观察,0.05%的秋水仙素处理24 h的诱变效果最佳,诱变率高达52%。用组织培养结合不定芽技术对变异株进行稳定,获得了稳定的多倍体。对变异株进行细胞学研究后发现,二倍体对照2n=2X=32,变异株2n=4X=64,为四倍体,另外还发现六倍体细胞的存在。

关键词: 马蹄莲, 多倍体, 秋水仙素, 染色体

Abstract:

Zantedeschia Aethiopia was treated with colchicines at different concentration and time duration in vitro. The results showed: the best effect was obtained by the treatment of 0.05﹪ colchicines with 24 hours, and its induction rate was 52%. Chromosome observation found that the chromosome number of treated plants was 64, and the chromosome number of the control was 32, indicating the tetraploid (2n =4x=64) of coloured Zantedeschia aethiopica is induced successfully.



Key words: Zantedeschia aethiopica, colchicines, polyploid, chromosome

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