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园艺学报 ›› 2008, Vol. 35 ›› Issue (3): 438-442.

• 研究简报 • 上一篇    下一篇

同源四倍体青花菜雄配子体的发育过程

万双粉1, 2 , 张蜀宁1* , 张 伟1 , 张红梅1 , 侯喜林1   

  1. 1 南京农业大学作物遗传与种质创新国家重点实验室, 南京210095; 2 云南省曲靖市麒麟区农业局, 云南曲靖
  • 收稿日期:2007-10-29 修回日期:2008-01-11 出版日期:2008-03-25 发布日期:2008-03-25
  • 通讯作者: 张蜀宁

Development Process of Male Gametophyte in Autotetraploid Broccoli

WAN Shuang-fen1, 2 , ZHANG Shu-ning1* , ZHANGWei1 , ZHANG Hong-mei1 , and HOU Xi-lin   

  1. (1 State Key Laboratory of Crop Genetics and Germ plasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2Agricultural Bureau of Qilin District in Qujing City, Qujing, Yunnan 655000, China)
  • Received:2007-10-29 Revised:2008-01-11 Online:2008-03-25 Published:2008-03-25
  • Contact: ZHANG Shu-ning

摘要: 利用“爱氏苏木精染色—冬青油透明”技术, 观察研究了同源四倍体青花菜( 2n = 4x = 36)
雄配子体的动态发育过程。结果表明: 同源四倍体青花菜与相应的二倍体在雄配子的整个发育过程上表现
相似, 细胞核经一次有丝分裂产生营养核与生殖核, 生殖核再经一次有丝分裂形成两个精核, 最终形成
3 - 细胞成熟花粉, 具4个或3个萌发孔; 雄配子体发育过程中异常频率为12114% , 败育主要发生在单核

关键词: 青花菜, 同源四倍体, 雄配子体发育

Abstract: This paper first reported the dynamic development of male gametophyte in autotetraploid broccoli using Ehrlichps hematoxylin staining-methyl salicylate clearing technique. Results showed that the male gametophyte in autotetraploid broccoli shared the similar development with that in diploid broccoli, which could give rise to one vegetative cell and one generative cell by one miotic division, while the generative cell could divide into two sperms by the othermiotic division and the mature pollen was 3-celled with four or three apertures. Abnormality appeared mainly in uninuclete stage of male gametophyte development with an average frequency of 12.14%.

Key words: Brassica oleracea L. var. italica Plenck, broccoli, autotetrap loid, male gametophyte development

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