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园艺学报 ›› 2003, Vol. 30 ›› Issue (1): 15-18.

• 研究论文 • 上一篇    下一篇

草莓采后微粒体膜Ca2+-ATPase 活性与膜脂过氧化水平

樊秀彩1,2;关军锋1*;张继澍2;李广敏1   

  1. (1 河北省农林科学院农业物理生理生化研究所, 石家庄050051 ;2西北农林科技大学生命科学学院, 杨凌712100)
  • 收稿日期:2002-04-25 修回日期:2002-06-23 出版日期:2003-02-25 发布日期:2003-02-25

Microsomal Membrane Ca2+-ATPase Activity and Lipid Peroxidation Levelduring Senescence of Postharvest Strawberry Fruit

Fan Xiucai1,2;Guan Junfeng1;Zhang Jishu2;Li Guangmin1   

  1. (1 Institute of Agro-Physics , Plant Physiology and Biochemistry , Hebei Academy of Agricultural and Forestry Sciences , Shijiazhuang 050051 , China ;2 Northwestern Science and Technology University of Agricultural and Forestry , Yangling 712100 , China)
  • Received:2002-04-25 Revised:2002-06-23 Online:2003-02-25 Published:2003-02-25

摘要: 测定了常温(25 ℃) 和低温(4 ℃) 贮藏的草莓果实中微粒体膜Ca2+-ATPase 酶(Ca2+-ATPase)与超氧化物歧化酶(SOD) 活性、超氧阴离子(O -·2 ) 产生速率和丙二醛(MDA) 含量的变化。结果显示,随果实衰老, 低温贮藏的果实微粒体膜Ca2+-ATPase总活性和质膜、线粒体膜及液泡膜上的Ca2+-ATPase 活性, 微粒体膜SOD 活性和O-·2 产生速率呈先升高后降低的趋势, MDA 含量变化与之相反; 常温贮藏的果实Ca2+-ATPase活性和O-·2产生速率变化较为迅速, 与低温类似, 而SOD 活性逐步下降, MDA 含量不断升高。这些表明, 草莓果实衰老与细胞质内Ca2+-稳态的破坏和膜脂过氧化作用的加强有密切关系。

关键词: 草莓, 果实, 衰老, 微粒体膜, Ca2+-ATPase, 膜脂过氧化

Abstract: Changes in activities of Ca2+-ATPase and SOD , O-·2 production rate and MDA content in microsomal membrane of strawberry fruit stored at normal temperature (25 ℃) and cold temperature (4 ℃) were investigated. The results showed that the activities of total microsomal membrane Ca2+-ATPase, plasmolemma Ca2+-ATPase , mitochondria-Ca2+-ATPase and tonoplast-Ca2+-ATPase , and of SOD , and O-·2 production rate all increased at first and then decreased , and MDA content changed in the contrary trend during senescence in fruit stored at 4 ℃. The changing trends in Ca2+-ATPase activities and O-·2 production rate of microsomal membrane in fruit stored at 25 ℃were consistent with the fruit stored at 4 ℃. However , a gradual decrease in SOD activity and increase in MDA content were found during room temperature storage. It is suggested that the fruit senescence was closely correlated with the damage of cytoplasmic Ca2+ homeostasis and the enhancement of membrane lipid
peroxidation in strawberry fruit

Key words: Strawberry, Fruit, Senescence, Microsomal membrane, Ca2+-ATPase, Lipid peroxidation

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