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园艺学报 ›› 2022, Vol. 49 ›› Issue (11): 2455-2470.doi: 10.16420/j.issn.0513-353x.2021-0876

• 研究报告 • 上一篇    下一篇

基于SLAF-seq的茶组子房室数不稳定居群种质亲缘关系分析

吴致君1,5, 李伟2, 王兴华3, 张成2, 蒋勋4, 黎盛1, 罗理勇1,5, 孙康1,5,*(), 曾亮1,5,*()   

  1. 1西南大学食品科学学院,重庆 400715
    2重庆市南川区农业特色产业发展中心,重庆 408400
    3云南农业大学茶学院,昆明 650201
    4云南普洱市茶叶科学研究所,云南普洱 665000
    5西南大学茶叶研究所,重庆 400715
  • 收稿日期:2021-12-02 修回日期:2022-06-13 出版日期:2022-11-25 发布日期:2022-11-25
  • 通讯作者: 孙康,曾亮 E-mail:sunkang@swu.edu.cn;zengliangbaby@126.com
  • 基金资助:
    重庆市技术创新与应用发展专项面上项目(cstc2020jscx-msxmX0032);重庆市技术创新与应用发展专项重点项目(cstc2021jscx- gksbX0016);重庆市技术创新与应用发展专项重点项目(cstc2020jscx-tpyzxX0009);国家自然科学基金项目(31800586);西南大学种质创制专项研究项目资助;北碚区基础研究与前沿探索项目(2020-25)

Genetic Relationship Analysis of the Population Germplasms of Section Thea with Unstable Ovary Locule Number Based on SLAF-seq

WU Zhijun1,5, LI Wei2, WANG Xinghua3, ZHANG Cheng2, JIANG Xun4, LI Sheng1, LUO Liyong1,5, SUN Kang1,5,*(), ZENG Liang1,5,*()   

  1. 1College of Food Science,Southwest University,Chongqing 400715,China
    2Nanchuan District’s Agricultural Characteristic Industry Development Center of Chongqing Municipality,Chongqing 408400,China
    3Tea College,Yunnan Agricultural University,Kunming 650201,China
    4Tea Science Research Institute of Pu-erh City of Yunnan Province,Pu’er,Yunnan 665000,China
    5Tea Research Institute,Southwest University,Chongqing 400715,China
  • Received:2021-12-02 Revised:2022-06-13 Online:2022-11-25 Published:2022-11-25
  • Contact: SUN Kang,ZENG Liang E-mail:sunkang@swu.edu.cn;zengliangbaby@126.com

摘要:

为了进一步探究子房室数不稳定(子房无毛、3 ~ 5室、花柱3 ~ 5裂)茶组(section Thea)居群种质(UONG)的亲缘关系,明确居群内个体的变异是否引起了种间变异,以重庆、云南及其他部分省份87个山茶属茶组和非茶组植物(外类群对照)种质为材料,利用SLAF-seq技术进行简化基因组测序和分子标记分析。测序共获得2 804 071个SALF标签,平均测序深度为11.73×。通过SALF标签比对参考基因组,共获得25 069 219个群体SNP分子标记。基于高一致性SNP数据,系统发育树、遗传结构和PCA分析等方法均将调查种质分为3种类型,即云南等的茶组和非茶组种质(Ⅰ类)、重庆大茶树居群种质(Ⅱ类)和多个省份来源的茶系(常见栽培种和‘川小种’)种质(Ⅲ类)。Ⅰ类种质中茶组植物,包含了五室茶系、五柱茶系、秃房茶系和茶系种质。Ⅱ类种质由UONG和茶系种质(形态特征介于UONG与常见茶系种质之间)组成。此外,遗传分化系数FST表明,Ⅱ类种质与其他几类种质遗传分化程度最高(> 0.3),Ⅰ类与Ⅲ类种质之间也存在较高的遗传分化(> 0.2)。简化基因组测序分类结果和FST值表明,调查的UONG为一类相对独立种质,居群内个体的变异并未引起种间变异。因此,将该类种质归入到一个种下的处理较为合理。此外,在重庆地区存在一类与UONG遗传结构交叉的茶系种质,可能为自然远缘杂交后代。

关键词: 茶组, 子房室数, 亲缘关系, SLAF-seq, SNP

Abstract:

In order to further explore the genetic relationship of the population germplasms of section Thea with unstable ovary locule number(ovary glabrous,3-5 loculed,style 3-5 lobed)(UONG),and to clarify whether the variation of individuals in the population caused interspecific variation,the simplified genome sequencing and molecular marker analysis of 87 germplasms of section Thea and other sections(outgroup comparison)of genus Camellia from Chongqing,Yunnan,and other provinces of China were carried out by SLAF-seq technology. A total of 2 804 071 SLAF labels were obtained by sequencing,and the average sequencing depth was 11.73×. A total of 25 069 219 SNP molecular markers were obtained by comparing the reference genome with SALF labels. Based on high consistency SNP data,using the methods of phylogenetic tree,genetic structure,and PCA analysis,the investigated germplasms were basically divided into three types,namely,the germplasms of section Thea and other sections of genus Camellia from Yunnan and other provinces(ClassⅠ),the population germplasms of big tea tree in Chongqing(ClassⅡ),and the Ser. Sinenses germplasms(common cultivated varieties and‘Chuanxiaozhong’)from multiple provinces(Class Ⅲ). The section Thea germplasms of ClassⅠwere composed of Ser. Guinquelocularis,Ser. Pentastyla,Ser. Gymnogynae,and Ser. Sinenses. ClassⅡ included UONG and Ser. Sinenses(morphological characteristics are between UONG and common Ser. Sinenses). Moreover,the FST values of genetic differentiation coefficient showed that the degree of genetic differentiation between ClassⅡgermplasms and other types of germplasms was the highest(> 0.3),and there was also high genetic differentiation between ClassⅠand Class Ⅲgermplasms(> 0.2). The classification results and FST values of simplified genome sequencing showed that the investigated UONG are relatively independent and the variation of individuals within the population did not cause interspecific variation. Therefore,it is reasonable to classify these germplasms into one species. Moreover,there is a type of Ser. Sinenses germplasms with cross genetic structure of UONG in Chongqing,which may be the offspring of natural distant hybridization.

Key words: section Thea, ovary locule number, genetic relationship, SLAF-seq, SNP

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