野生蛹虫草分化株子实体表型性状的分离

doi:10.16420/j.issn.0513-353x.2021-0683

摘要/Abstract

摘要：

对采集于辽宁西丰、辽宁大石桥的3株（C5、D1、D5）野生蛹虫草后代6个分化株（C5-1、C5-2、D1-1、D1-2、D5-1、D5-2）进行菌落形态、农艺性状、营养成分对比，并利用ISSR分子标记技术进行遗传差异性分析。结果表明，来源于同一株野生蛹虫草子实体的分化株菌落生长速度、子实体高度、子实体密度多数有显著差异。ISSR标记分析表明同一株野生蛹虫草的分化株D1-1与D1-2、D5-1与D5-2基因型差异较大。来源于同一株野生蛹虫草的分化株虫草素、腺苷含量差异显著。

关键词: 蛹虫草, 表型性状, 虫草素, 腺苷, 分化株

Abstract:

Six Cordyceps militaris（C5-1，C5-2，D1-1，D1-2，D1-2，D5-1，D5-2）which were the offspring of three（C5，D1，D5）wild C. militaris which were collected from Xifeng and Dashiqiao of Liaoning Province were compared in terms of colony morphology，agronomic traits and nutritional composition. Then，the genetic differences were analyzed by ISSR molecular markers. The results showed that there were significant differences in growth rate，fruiting body height and density among the differentiated strains from the same wild cordyceps militaris fruiting body. It can be seen from the ISSR marker analysis of the differentiated strains that the genotypes of D1-1 and D1-2，D5-1 and D5-2 from the same wild cordyceps militaris were significantly different. Moreover，there were significant differences in the content of cordycepin and adenosine among the differentiated cordyceps militaris strains from the same wild cordyceps militaris strains.

Key words: Cordyceps militaris, phenotypic trait, cordycepin, adenosine, differentiated strain

中图分类号:

S646
Q 939.9

肖军, 陈珣, 肇莹, 马晓颖, 龚娜, 刘国丽. 野生蛹虫草分化株子实体表型性状的分离[J]. 园艺学报, 2022, 49(11): 2449-2454.

XIAO Jun, CHEN Xun, ZHAO Ying, MA Xiaoying, GONG Na, LIU Guoli. Isolation of Phenotypic Traits from Wild Cordyceps militaris Fruitbody[J]. Acta Horticulturae Sinica, 2022, 49(11): 2449-2454.

图/表 6

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引物编号 Primer	核苷酸序列（5′-3′） Sequence	引物编号 Primer	核苷酸序列（5′-3′） Sequence
ISSR1	CTCCTCCTCCTCSC	ISSR6	DHBCGACGACGACGACGA
ISSR2	BDBACAACAACAACAACA	ISSR7	AGAGAGAGAGAGAGAGT
ISSR3	CACACACACACACACAWG	ISSR8	GAGAGAGAGAGAGAGAC
ISSR4	CACCACCACCACSC	ISSR9	CTCTCTCTCTCTCTCTG
ISSR5	VDHTCGTCGTCGTCGTCG	ISSR10	CACACACACACACACAG

引物编号 Primer	核苷酸序列（5′-3′） Sequence	引物编号 Primer	核苷酸序列（5′-3′） Sequence
ISSR1	CTCCTCCTCCTCSC	ISSR6	DHBCGACGACGACGACGA
ISSR2	BDBACAACAACAACAACA	ISSR7	AGAGAGAGAGAGAGAGT
ISSR3	CACACACACACACACAWG	ISSR8	GAGAGAGAGAGAGAGAC
ISSR4	CACCACCACCACSC	ISSR9	CTCTCTCTCTCTCTCTG
ISSR5	VDHTCGTCGTCGTCGTCG	ISSR10	CACACACACACACACAG

分化株 Differentiated strain	暗培养7 d The first 7 days without light	满皿Full dish
分化株 Differentiated strain	暗培养7 d The first 7 days without light	+ 见光培养 + Light	+ 暗培养 + No light
C5-1	3.31 ± 0.08 ab	2.76 ± 0.22 a	3.17 ± 0.14 ab
C5-2	3.52 ± 0.14 b	3.34 ± 0.14 b	3.24 ± 0.08 ab
D1-1	3.29 ± 0.14 ab	3.12 ± 0.22 ab	3.01 ± 0.29 a
D1-2	3.18 ± 0.08 ab	3.21 ± 0.08 b	3.23 ± 0.08 ab
D5-1	3.93 ± 0.28 c	3.29 ± 0.15 b	3.46 ± 0.22 b
D5-2	3.11 ± 0.16 a	3.02 ± 0.16 ab	3.14 ± 0.16 ab

分化株 Differentiated strain	暗培养7 d The first 7 days without light	满皿Full dish
分化株 Differentiated strain	暗培养7 d The first 7 days without light	+ 见光培养 + Light	+ 暗培养 + No light
C5-1	3.31 ± 0.08 ab	2.76 ± 0.22 a	3.17 ± 0.14 ab
C5-2	3.52 ± 0.14 b	3.34 ± 0.14 b	3.24 ± 0.08 ab
D1-1	3.29 ± 0.14 ab	3.12 ± 0.22 ab	3.01 ± 0.29 a
D1-2	3.18 ± 0.08 ab	3.21 ± 0.08 b	3.23 ± 0.08 ab
D5-1	3.93 ± 0.28 c	3.29 ± 0.15 b	3.46 ± 0.22 b
D5-2	3.11 ± 0.16 a	3.02 ± 0.16 ab	3.14 ± 0.16 ab

分化株Differentiated strain	数量Number	高度/cm Height
C5-1	60.33 ± 2.94 a	5.32 ± 0.22 a
C5-2	102.92 ± 4.97 b	5.16 ± 0.14 a
D1-1	144.50 ± 4.55 d	5.62 ± 0.28 a
D1-2	118.33 ± 2.16 c	8.80 ± 0.22 c
D5-1	108.00 ± 2.83 b	7.52 ± 0.16 b
D5-2	187.27 ± 5.10 e	9.56 ± 0.29 d