园艺学报 ›› 2022, Vol. 49 ›› Issue (1): 175-186.doi: 10.16420/j.issn.0513-353x.2021-0312
陈明堃1, 陈璐1, 孙维红2, 马山虎1, 兰思仁1, 彭东辉1, 刘仲健1, 艾叶1,*()
收稿日期:
2021-05-09
修回日期:
2021-11-26
出版日期:
2022-01-25
发布日期:
2022-01-24
通讯作者:
艾叶
E-mail:aiyefafu@163.com
基金资助:
CHEN Mingkun1, CHEN Lu1, SUN Weihong2, MA Shanhu1, LAN Siren1, PENG Donghui1, LIU Zhongjian1, AI Ye1,*()
Received:
2021-05-09
Revised:
2021-11-26
Online:
2022-01-25
Published:
2022-01-24
Contact:
AI Ye
E-mail:aiyefafu@163.com
摘要:
研究记录了311份建兰(Cymbidium ensifolium)种质资源的萼片、花瓣、唇瓣颜色以及叶艺、瓣型和唇瓣斑点等质量性状的表型特征,并对其进行赋值和统计分析;利用22对SSR荧光引物对311份建兰种质资源进行PCR扩增,分析其遗传多样性;利用Structure软件分析建兰种质资源的群体结构,采用主成分分析和聚类分析进行验证;运用Core Finder软件和t检验分析并筛选核心种质。结果表明:311份建兰种质资源的6个质量性状的表型遗传多样性均大于0.5,表明建兰种质资源的表型具有丰富的遗传变异;22对SSR荧光引物共检测到171个等位基因(Na),平均有效等位基因数(Ne)为2.729,Shannon’s信息指数(Ⅰ)为1.026,表明建兰种质资源具有丰富的遗传多样性;Structure软件分析结果显示最优的群体数K值为3,群体间有少量种质混杂;群体结构分析将建兰种质资源分为3个类群,主成分分析和聚类分析进一步验证了其分类的可靠性。本研究中构建的建兰核心种质51份占原有种质资源的16.4%,等位基因数(Na)保留率为100%,有效等位基因数(Ne)保留率为130%,Shannon’s信息指数(Ⅰ)保留率为124%。通过t检验和主坐标分析发现,核心种质与原有种质的遗传多样性无显著差异,说明构建的51份核心种质能最大程度代表建兰种质资源的遗传多样性。
中图分类号:
陈明堃, 陈璐, 孙维红, 马山虎, 兰思仁, 彭东辉, 刘仲健, 艾叶. 建兰种质资源遗传多样性分析及核心种质构建[J]. 园艺学报, 2022, 49(1): 175-186.
CHEN Mingkun, CHEN Lu, SUN Weihong, MA Shanhu, LAN Siren, PENG Donghui, LIU Zhongjian, AI Ye. Genetic Diversity Analysis and Core Collection of Cymbidium ensifolium Germplasm Resources[J]. Acta Horticulturae Sinica, 2022, 49(1): 175-186.
引物 Primer | 正向引物(5′-3′) Forward primer | 反向引物(5′-3′) Reverse primer | 扩增范围/bp Amplification range |
---|---|---|---|
JL6 | TGGGCGACAGATCGAGTTC | ACATGGACCACAGCATTCC | 335 ~ 366 |
JL8 | TGCTGGAATACATGCGAGAC | GTTTGCCGAAGCCAGTGC | 140 ~ 165 |
JL9 | GCTTTATGCGACATCTGCG | CGTCGGTTCCATGCACATC | 175 ~ 225 |
JL14 | TATGCGTCTCTCCCAACCG | AAGCTAGTGGCCTTTGGTG | 225 ~ 260 |
JL15 | AGAGGGCCACCTGCTTTC | GCCAATTGCCAGATGGACG | 290 ~ 320 |
JL16 | GGTGGGTTAGACCAGCTCC | TCCTCAAGGCAAAGCTCCC | 185 ~ 190 |
JL17 | CCCAACGCAGAACGATAGC | CGGTGGCACAAATGGAACG | 205 ~ 215 |
JL18 | AGACAGAGAGTCCCTAAAGGC | CAGGGATGTTAAGTGGGCTG | 430 ~ 455 |
JL19 | AGTATTGGACCCTCCAGGC | AGAGGATCATGGTGTTAGGC | 370 ~ 395 |
JL20 | GACGACATATCGCGTTCGG | CTCAGCCACACCCAAGAGG | 220 ~ 240 |
JL21 | GGAGCTGCATACGCAAGTG | AGCTTCTCACTGCCTCCAG | 460 ~ 500 |
JL22 | TGAAACGGTTGGCTCTAGTTC | AGCAAGCACTGACCTGAAAC | 260 ~ 290 |
JL24 | AACGCCATGTCCAATACCC | GGAGGGCTTATTTGCAGCG | 365 ~ 430 |
JL25 | AAACGAAGGGCTGGAAGTC | TTTGACATCGGGAAGTGGC | 255 ~ 270 |
JL28 | ATCTTCCCTCCACATCGGC | TGGAGAAGAGTCGACCAGC | 235 ~ 260 |
JL29 | AACTGACAAGCATCTGCAAG | CTGCTGCATTGGCCTTACC | 265 ~ 285 |
JL30 | TTTGTGGCAGTGGAAAGCG | TGATACCAATGGCAAGGCG | 270 ~ 300 |
JL31 | CACAGCAGCTCACAATCCTG | TACAGCCCTGTTTACCGCC | 270 ~ 290 |
JL34 | AAGAGGCACTGCAAGACCC | CGTTCCAGCAACCCATAGC | 425 ~ 450 |
JL38 | GCCTTACACATCCCTTCCAAC | TGCCTGCTGATAGTTTGCC | 400 ~ 420 |
JL41 | CTTCCTCCACCTGGATCGC | CTGCCGATCAATCCGAGAC | 170 ~ 180 |
JL43 | CTCGGTTCATTTGCAGCCC | GGGTGGGTATGGCGAAATC | 340 ~ 360 |
表1 SSR荧光引物及其序列
Table 1 Sequences of SSR fluorescent primers
引物 Primer | 正向引物(5′-3′) Forward primer | 反向引物(5′-3′) Reverse primer | 扩增范围/bp Amplification range |
---|---|---|---|
JL6 | TGGGCGACAGATCGAGTTC | ACATGGACCACAGCATTCC | 335 ~ 366 |
JL8 | TGCTGGAATACATGCGAGAC | GTTTGCCGAAGCCAGTGC | 140 ~ 165 |
JL9 | GCTTTATGCGACATCTGCG | CGTCGGTTCCATGCACATC | 175 ~ 225 |
JL14 | TATGCGTCTCTCCCAACCG | AAGCTAGTGGCCTTTGGTG | 225 ~ 260 |
JL15 | AGAGGGCCACCTGCTTTC | GCCAATTGCCAGATGGACG | 290 ~ 320 |
JL16 | GGTGGGTTAGACCAGCTCC | TCCTCAAGGCAAAGCTCCC | 185 ~ 190 |
JL17 | CCCAACGCAGAACGATAGC | CGGTGGCACAAATGGAACG | 205 ~ 215 |
JL18 | AGACAGAGAGTCCCTAAAGGC | CAGGGATGTTAAGTGGGCTG | 430 ~ 455 |
JL19 | AGTATTGGACCCTCCAGGC | AGAGGATCATGGTGTTAGGC | 370 ~ 395 |
JL20 | GACGACATATCGCGTTCGG | CTCAGCCACACCCAAGAGG | 220 ~ 240 |
JL21 | GGAGCTGCATACGCAAGTG | AGCTTCTCACTGCCTCCAG | 460 ~ 500 |
JL22 | TGAAACGGTTGGCTCTAGTTC | AGCAAGCACTGACCTGAAAC | 260 ~ 290 |
JL24 | AACGCCATGTCCAATACCC | GGAGGGCTTATTTGCAGCG | 365 ~ 430 |
JL25 | AAACGAAGGGCTGGAAGTC | TTTGACATCGGGAAGTGGC | 255 ~ 270 |
JL28 | ATCTTCCCTCCACATCGGC | TGGAGAAGAGTCGACCAGC | 235 ~ 260 |
JL29 | AACTGACAAGCATCTGCAAG | CTGCTGCATTGGCCTTACC | 265 ~ 285 |
JL30 | TTTGTGGCAGTGGAAAGCG | TGATACCAATGGCAAGGCG | 270 ~ 300 |
JL31 | CACAGCAGCTCACAATCCTG | TACAGCCCTGTTTACCGCC | 270 ~ 290 |
JL34 | AAGAGGCACTGCAAGACCC | CGTTCCAGCAACCCATAGC | 425 ~ 450 |
JL38 | GCCTTACACATCCCTTCCAAC | TGCCTGCTGATAGTTTGCC | 400 ~ 420 |
JL41 | CTTCCTCCACCTGGATCGC | CTGCCGATCAATCCGAGAC | 170 ~ 180 |
JL43 | CTCGGTTCATTTGCAGCCC | GGGTGGGTATGGCGAAATC | 340 ~ 360 |
性状 Trait | 赋值(频率) Assignment value(Frequency) | 多样性 指数(I) Diversity index | |||||||
---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||
萼片颜色 Sepal color | 黄绿 Yellowish- green(82.32%) | 复色 Multiple (4.50%) | 黄 Yellow (1.61%) | 粉红 Pink (4.82%) | 紫红 Fuchsia (1.61%) | 白 White (5.14%) | 0.7315 | ||
花瓣颜色 Petal color | 黄绿 Yellowish- green(70.09%) | 黄 Yellow (11.25%) | 复色 Multiple (3.86%) | 粉红 Pink (5.47%) | 紫红 Fuchsia (1.61%) | 白 White (7.72%) | 1.0435 | ||
唇瓣颜色 Lip color | 黄绿 Yellowish- green(49.84%) | 黄 Yellow (40.84%) | 白 White (8.36%) | 红 Red (0.64%) | 紫红 Fuchsia (0.32%) | 0.9712 | |||
叶艺 Leaf art | 正常叶 Normal leaf (79.74%) | 线艺 Thread art (8.36%) | 斑艺 Spot art (0.64%) | 多重叶艺 Multiple leaf art (5.15%) | 矮种 Dwarf species (2.57%) | 行龙 Xing long (1.93%) | 水晶艺 Crystal art (1.29%) | 叶蝶 Butterfly leaf (0.32%) | 0.8179 |
瓣型 Petal type | 竹叶瓣 Bamboo-like (42.44%) | 奇花 Peculiar flower(19.94%) | 水仙瓣 Narcissus-like (3.86%) | 荷瓣 Lotus-like (19.61%) | 梅瓣 Plum-like (14.15%) | 1.4070 | |||
唇瓣斑点 Lip spot | 有 Yes (76.85%) | 无 None (23.15%) | 0.5411 |
表2 建兰种质资源质量性状赋值及分布频率
Table 2 The assigning values of qualitative traits of Cymbidium ensifolium germplasm resources and their distribution frequency
性状 Trait | 赋值(频率) Assignment value(Frequency) | 多样性 指数(I) Diversity index | |||||||
---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||
萼片颜色 Sepal color | 黄绿 Yellowish- green(82.32%) | 复色 Multiple (4.50%) | 黄 Yellow (1.61%) | 粉红 Pink (4.82%) | 紫红 Fuchsia (1.61%) | 白 White (5.14%) | 0.7315 | ||
花瓣颜色 Petal color | 黄绿 Yellowish- green(70.09%) | 黄 Yellow (11.25%) | 复色 Multiple (3.86%) | 粉红 Pink (5.47%) | 紫红 Fuchsia (1.61%) | 白 White (7.72%) | 1.0435 | ||
唇瓣颜色 Lip color | 黄绿 Yellowish- green(49.84%) | 黄 Yellow (40.84%) | 白 White (8.36%) | 红 Red (0.64%) | 紫红 Fuchsia (0.32%) | 0.9712 | |||
叶艺 Leaf art | 正常叶 Normal leaf (79.74%) | 线艺 Thread art (8.36%) | 斑艺 Spot art (0.64%) | 多重叶艺 Multiple leaf art (5.15%) | 矮种 Dwarf species (2.57%) | 行龙 Xing long (1.93%) | 水晶艺 Crystal art (1.29%) | 叶蝶 Butterfly leaf (0.32%) | 0.8179 |
瓣型 Petal type | 竹叶瓣 Bamboo-like (42.44%) | 奇花 Peculiar flower(19.94%) | 水仙瓣 Narcissus-like (3.86%) | 荷瓣 Lotus-like (19.61%) | 梅瓣 Plum-like (14.15%) | 1.4070 | |||
唇瓣斑点 Lip spot | 有 Yes (76.85%) | 无 None (23.15%) | 0.5411 |
引物 Primer | Na 等位基因数 Number of alleles | Ne 有效等位基因数 Number of effective alleles | Ho 观测杂合度 Observed heterozygosity | He 期望杂合度 Expected heterozygosity | I Shannon’s信息指数 Shannon’s information index |
---|---|---|---|---|---|
JL6 | 10 | 1.390 | 0.226 | 0.280 | 0.657 |
JL8 | 8 | 2.433 | 0.461 | 0.589 | 1.207 |
JL9 | 14 | 4.595 | 0.511 | 0.782 | 1.830 |
JL14 | 10 | 3.783 | 0.680 | 0.736 | 1.509 |
JL15 | 11 | 1.514 | 0.300 | 0.339 | 0.803 |
JL16 | 3 | 1.063 | 0.053 | 0.059 | 0.150 |
JL17 | 6 | 2.661 | 0.197 | 0.624 | 1.079 |
JL18 | 3 | 1.928 | 0.455 | 0.481 | 0.725 |
JL19 | 4 | 2.197 | 0.316 | 0.545 | 0.870 |
JL20 | 9 | 4.900 | 0.354 | 0.796 | 1.721 |
JL21 | 12 | 2.264 | 0.218 | 0.558 | 1.274 |
JL22 | 5 | 2.170 | 0.485 | 0.539 | 0.983 |
JL24 | 23 | 10.189 | 0.676 | 0.902 | 2.602 |
JL25 | 6 | 1.948 | 0.259 | 0.487 | 0.825 |
JL28 | 5 | 1.100 | 0.049 | 0.091 | 0.241 |
JL29 | 4 | 1.350 | 0.191 | 0.259 | 0.457 |
JL30 | 10 | 5.592 | 0.287 | 0.821 | 1.918 |
JL31 | 5 | 1.970 | 0.127 | 0.492 | 0.848 |
JL34 | 7 | 1.708 | 0.327 | 0.414 | 0.844 |
JL38 | 9 | 2.269 | 0.458 | 0.559 | 1.168 |
JL41 | 4 | 1.965 | 0.331 | 0.491 | 0.710 |
JL43 | 3 | 1.057 | 0.049 | 0.054 | 0.144 |
均值Average | 7.773 | 2.729 | 0.319 | 0.496 | 1.026 |
表3 建兰种质资源遗传多样性信息
Table 3 The genetic diversity information of Cymbidium ensifolium germplasm resources
引物 Primer | Na 等位基因数 Number of alleles | Ne 有效等位基因数 Number of effective alleles | Ho 观测杂合度 Observed heterozygosity | He 期望杂合度 Expected heterozygosity | I Shannon’s信息指数 Shannon’s information index |
---|---|---|---|---|---|
JL6 | 10 | 1.390 | 0.226 | 0.280 | 0.657 |
JL8 | 8 | 2.433 | 0.461 | 0.589 | 1.207 |
JL9 | 14 | 4.595 | 0.511 | 0.782 | 1.830 |
JL14 | 10 | 3.783 | 0.680 | 0.736 | 1.509 |
JL15 | 11 | 1.514 | 0.300 | 0.339 | 0.803 |
JL16 | 3 | 1.063 | 0.053 | 0.059 | 0.150 |
JL17 | 6 | 2.661 | 0.197 | 0.624 | 1.079 |
JL18 | 3 | 1.928 | 0.455 | 0.481 | 0.725 |
JL19 | 4 | 2.197 | 0.316 | 0.545 | 0.870 |
JL20 | 9 | 4.900 | 0.354 | 0.796 | 1.721 |
JL21 | 12 | 2.264 | 0.218 | 0.558 | 1.274 |
JL22 | 5 | 2.170 | 0.485 | 0.539 | 0.983 |
JL24 | 23 | 10.189 | 0.676 | 0.902 | 2.602 |
JL25 | 6 | 1.948 | 0.259 | 0.487 | 0.825 |
JL28 | 5 | 1.100 | 0.049 | 0.091 | 0.241 |
JL29 | 4 | 1.350 | 0.191 | 0.259 | 0.457 |
JL30 | 10 | 5.592 | 0.287 | 0.821 | 1.918 |
JL31 | 5 | 1.970 | 0.127 | 0.492 | 0.848 |
JL34 | 7 | 1.708 | 0.327 | 0.414 | 0.844 |
JL38 | 9 | 2.269 | 0.458 | 0.559 | 1.168 |
JL41 | 4 | 1.965 | 0.331 | 0.491 | 0.710 |
JL43 | 3 | 1.057 | 0.049 | 0.054 | 0.144 |
均值Average | 7.773 | 2.729 | 0.319 | 0.496 | 1.026 |
类群 Group | 各类群种质数量 Number of germplasm in each group | 种质数量(频率) Frequency of germplasm numbers | |||
---|---|---|---|---|---|
Q < 0.6 | 0.6 ≤ Q < 0.8 | 0.8 ≤ Q < 0.9 | Q ≥ 0.9 | ||
Ⅰ | 82 | 2(2.4%) | 9(11.0%) | 11(13.4%) | 60(73.2%) |
Ⅱ | 64 | 7(10.7%) | 12(18.9%) | 9(14.1%) | 36(56.3%) |
Ⅲ | 165 | 16(9.7%) | 26(15.8%) | 24(14.5%) | 99(60.0%) |
合计Total | 311 | 25(8.0%) | 47(15.1%) | 44(14.1%) | 195(62.8%) |
表4 各类群Q值分布
Table 4 Distribution of Q-value of three groups
类群 Group | 各类群种质数量 Number of germplasm in each group | 种质数量(频率) Frequency of germplasm numbers | |||
---|---|---|---|---|---|
Q < 0.6 | 0.6 ≤ Q < 0.8 | 0.8 ≤ Q < 0.9 | Q ≥ 0.9 | ||
Ⅰ | 82 | 2(2.4%) | 9(11.0%) | 11(13.4%) | 60(73.2%) |
Ⅱ | 64 | 7(10.7%) | 12(18.9%) | 9(14.1%) | 36(56.3%) |
Ⅲ | 165 | 16(9.7%) | 26(15.8%) | 24(14.5%) | 99(60.0%) |
合计Total | 311 | 25(8.0%) | 47(15.1%) | 44(14.1%) | 195(62.8%) |
图4 建兰种质资源邻接法聚类分析 绿色部分代表类群Ⅰ材料、红色部分代表类群Ⅱ材料、蓝色部分代表类群Ⅲ材料。
Fig. 4 The cluster analysis of Cymbidium ensifolium germplasm resources based on the neighbor-joining method The green part represents groupⅠ materials,the red part represents groupⅡ materials,and the blue part represents group Ⅲ materials.
序号 Number | 种质(瓣型) Germplasm(Petal type) | 序号 Number | 种质(瓣型) Germplasm(Petal type) | 序号 Number | 种质(瓣型) Germplasm(Petal type) | |
---|---|---|---|---|---|---|
1 | 中国龙Zhongguolong (荷瓣Lotus-like) | 18 | 桃腮素 Taosaisu (竹叶瓣 Bamboo-like) | 35 | 越南四季 Yuenan Siji (竹叶瓣 Bamboo-like) | |
2 | 彩云荷 Caiyunhe (荷瓣Lotus-like) | 19 | 日月潭之恋 Riyuetan Zhilian (竹叶瓣 Bamboo-like) | 36 | 连城大荷素 Liancheng Dahesu (竹叶瓣 Bamboo-like) | |
3 | 云南下山 Yunnan Xiashan (竹叶瓣Bamboo-like) | 20 | 素君荷 Sujunhe (荷瓣 Lotus-like) | 37 | 观音素 Guanyinsu (竹叶瓣 Bamboo-like) | |
4 | 五岳奇蝶 Wuyue Qidie (奇花 Peculiar flower) | 21 | 君临天下 Junlin Tianxia (荷瓣 Lotus-like) | 38 | 翠衣仙子 Cuiyi Xianzi (水仙瓣 Narcissus-like) | |
5 | 四季华光蝶 Siji Huaguangdie (奇花 Peculiar flower) | 22 | 黄金彩梅 Huangjin Caimei (梅瓣 Plum-like) | 39 | 朝阳三星 Zhaoyang Sanxing (奇花 Peculiar flower) | |
6 | 龙凤大彩梅 Longfeng Dacaimei (梅瓣 Plum-like) | 23 | 建兰水晶艺 Jianlan Shuijingyi (竹叶瓣 Bamboo-like) | 40 | 蝴蝶王 Hudiewang (奇花 Peculiar flower) | |
7 | 下山素 Xiashansu (竹叶瓣 Bamboo-like) | 24 | 大满贯 Damanguan (竹叶瓣 Bamboo-like) | 41 | 建兰红芽 Jianlan Hongya (竹叶瓣 Bamboo-like) | |
8 | 大叶铁骨 Daye Tiegu (竹叶瓣 Bamboo-like) | 25 | 四季罗汉 Siji Luohan (荷瓣 Lotus-like) | 42 | 老种荷仙 Laozhong Hexian (水仙瓣 Narcissus-like) | |
9 | 银边大贡 Yinbian Dagong (竹叶瓣 Bamboo-like) | 26 | 新君荷 Xinjunhe (荷瓣 Lotus-like) | 43 | 四季红宋梅 Siji Hongsongmei (梅瓣 Plum-like) | |
10 | 泸州荷仙 Luzhou Hexian (水仙瓣 Narcissus-like) | 27 | 立叶剑惠 Liye Jianhui (竹叶瓣 Bamboo-like) | 44 | 翡翠梅 Feicuimei (梅瓣 Plum-like) | |
11 | 火凤凰 Huofenghuang (奇花 Peculiar flower) | 28 | 蜘蛛瓣 Zhizhuban (奇花 Peculiar flower) | 45 | 大青 Daqing (竹叶瓣 Bamboo-like) | |
12 | 复兴奇蝶 Fuxing Qidie (奇花 Peculiar flower) | 29 | 海南鹅岭 Hainan Eling (竹叶瓣 Bamboo-like) | 46 | 红猫蕊蝶 Hongmao Ruidie (奇花 Peculiar flower) | |
13 | 桂荷三星 Guihe Sanxing (荷瓣Lotus-like) | 30 | 铁骨金龙 Tiegu Jinlong (竹叶瓣 Bamboo-like) | 47 | 南靖下山 Nanjng Xiashan (竹叶瓣 Bamboo-like) | |
14 | 峨眉弦 Emeixian (竹叶瓣 Bamboo-like) | 31 | 富士锦 Fushijin (竹叶瓣 Bamboo-like) | 48 | 仙山红 Xianshanhong (荷瓣 Lotus-like) | |
15 | 福建永定 Fujian Yongding (竹叶瓣 Bamboo-like) | 32 | 铁骨银针 Tiegu Yinzhen (竹叶瓣 Bamboo-like) | 49 | 红顶荷 Hongdinghe (荷瓣 Lotus-like) | |
16 | 仁化大荷素 Renhua Dahesu (荷瓣 Lotus-like) | 33 | 四季建兰 Siji Jianlan (竹叶瓣 Bamboo-like) | 50 | 黄一品 Huangyipin (梅瓣 Plum-like) | |
17 | 桃园三结义 Taoyuan Sanjieyi (奇花 Peculiar flower) | 34 | 四季素 Sijisu (竹叶瓣 Bamboo-like) | 51 | 八宝奇珍 Babao Qizhen (竹叶瓣 Bamboo-like) |
表5 建兰核心种质的信息
Table 5 Information of core collections of Cymbidium ensifolium
序号 Number | 种质(瓣型) Germplasm(Petal type) | 序号 Number | 种质(瓣型) Germplasm(Petal type) | 序号 Number | 种质(瓣型) Germplasm(Petal type) | |
---|---|---|---|---|---|---|
1 | 中国龙Zhongguolong (荷瓣Lotus-like) | 18 | 桃腮素 Taosaisu (竹叶瓣 Bamboo-like) | 35 | 越南四季 Yuenan Siji (竹叶瓣 Bamboo-like) | |
2 | 彩云荷 Caiyunhe (荷瓣Lotus-like) | 19 | 日月潭之恋 Riyuetan Zhilian (竹叶瓣 Bamboo-like) | 36 | 连城大荷素 Liancheng Dahesu (竹叶瓣 Bamboo-like) | |
3 | 云南下山 Yunnan Xiashan (竹叶瓣Bamboo-like) | 20 | 素君荷 Sujunhe (荷瓣 Lotus-like) | 37 | 观音素 Guanyinsu (竹叶瓣 Bamboo-like) | |
4 | 五岳奇蝶 Wuyue Qidie (奇花 Peculiar flower) | 21 | 君临天下 Junlin Tianxia (荷瓣 Lotus-like) | 38 | 翠衣仙子 Cuiyi Xianzi (水仙瓣 Narcissus-like) | |
5 | 四季华光蝶 Siji Huaguangdie (奇花 Peculiar flower) | 22 | 黄金彩梅 Huangjin Caimei (梅瓣 Plum-like) | 39 | 朝阳三星 Zhaoyang Sanxing (奇花 Peculiar flower) | |
6 | 龙凤大彩梅 Longfeng Dacaimei (梅瓣 Plum-like) | 23 | 建兰水晶艺 Jianlan Shuijingyi (竹叶瓣 Bamboo-like) | 40 | 蝴蝶王 Hudiewang (奇花 Peculiar flower) | |
7 | 下山素 Xiashansu (竹叶瓣 Bamboo-like) | 24 | 大满贯 Damanguan (竹叶瓣 Bamboo-like) | 41 | 建兰红芽 Jianlan Hongya (竹叶瓣 Bamboo-like) | |
8 | 大叶铁骨 Daye Tiegu (竹叶瓣 Bamboo-like) | 25 | 四季罗汉 Siji Luohan (荷瓣 Lotus-like) | 42 | 老种荷仙 Laozhong Hexian (水仙瓣 Narcissus-like) | |
9 | 银边大贡 Yinbian Dagong (竹叶瓣 Bamboo-like) | 26 | 新君荷 Xinjunhe (荷瓣 Lotus-like) | 43 | 四季红宋梅 Siji Hongsongmei (梅瓣 Plum-like) | |
10 | 泸州荷仙 Luzhou Hexian (水仙瓣 Narcissus-like) | 27 | 立叶剑惠 Liye Jianhui (竹叶瓣 Bamboo-like) | 44 | 翡翠梅 Feicuimei (梅瓣 Plum-like) | |
11 | 火凤凰 Huofenghuang (奇花 Peculiar flower) | 28 | 蜘蛛瓣 Zhizhuban (奇花 Peculiar flower) | 45 | 大青 Daqing (竹叶瓣 Bamboo-like) | |
12 | 复兴奇蝶 Fuxing Qidie (奇花 Peculiar flower) | 29 | 海南鹅岭 Hainan Eling (竹叶瓣 Bamboo-like) | 46 | 红猫蕊蝶 Hongmao Ruidie (奇花 Peculiar flower) | |
13 | 桂荷三星 Guihe Sanxing (荷瓣Lotus-like) | 30 | 铁骨金龙 Tiegu Jinlong (竹叶瓣 Bamboo-like) | 47 | 南靖下山 Nanjng Xiashan (竹叶瓣 Bamboo-like) | |
14 | 峨眉弦 Emeixian (竹叶瓣 Bamboo-like) | 31 | 富士锦 Fushijin (竹叶瓣 Bamboo-like) | 48 | 仙山红 Xianshanhong (荷瓣 Lotus-like) | |
15 | 福建永定 Fujian Yongding (竹叶瓣 Bamboo-like) | 32 | 铁骨银针 Tiegu Yinzhen (竹叶瓣 Bamboo-like) | 49 | 红顶荷 Hongdinghe (荷瓣 Lotus-like) | |
16 | 仁化大荷素 Renhua Dahesu (荷瓣 Lotus-like) | 33 | 四季建兰 Siji Jianlan (竹叶瓣 Bamboo-like) | 50 | 黄一品 Huangyipin (梅瓣 Plum-like) | |
17 | 桃园三结义 Taoyuan Sanjieyi (奇花 Peculiar flower) | 34 | 四季素 Sijisu (竹叶瓣 Bamboo-like) | 51 | 八宝奇珍 Babao Qizhen (竹叶瓣 Bamboo-like) |
种质 Collection | 种质数 Number of Collection | Na | Ne | I | Ho | He |
---|---|---|---|---|---|---|
原有种质 Initial collection | 311 | 7.773 | 2.729 | 1.026 | 0.319 | 0.496 |
核心种质 Core collection | 51(16%) | 7.773(100%) | 3.551(130%) | 1.268(124%) | 0.360(113%) | 0.573(116%) |
保留种质 Reserve collection | 260(84%) | 5.955(77%) | 2.585(95%) | 0.951(93%) | 0.308(97%) | 0.476(96%) |
t1 | 0.000 | -0.962 | -1.277 | -0.793 | -1.112 | |
t2 | 1.409 | 1.164 | 1.687 | 0.942 | 1.365 |
表6 建兰核心种质、保留种质和原有种质的遗传多样性对比
Table 6 Comparison of genetic diversity among Cymbidium ensifolium core collection,reserve collection and initial collection
种质 Collection | 种质数 Number of Collection | Na | Ne | I | Ho | He |
---|---|---|---|---|---|---|
原有种质 Initial collection | 311 | 7.773 | 2.729 | 1.026 | 0.319 | 0.496 |
核心种质 Core collection | 51(16%) | 7.773(100%) | 3.551(130%) | 1.268(124%) | 0.360(113%) | 0.573(116%) |
保留种质 Reserve collection | 260(84%) | 5.955(77%) | 2.585(95%) | 0.951(93%) | 0.308(97%) | 0.476(96%) |
t1 | 0.000 | -0.962 | -1.277 | -0.793 | -1.112 | |
t2 | 1.409 | 1.164 | 1.687 | 0.942 | 1.365 |
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